Recombinant Drosophila melanogaster Vitellogenin-1 (Yp1)

Code CSB-YP365787DLU
MSDS
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Source Yeast
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Code CSB-EP365787DLU
MSDS
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Source E.coli
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Code CSB-EP365787DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP365787DLU
MSDS
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Source Baculovirus
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Code CSB-MP365787DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Yp1
Uniprot No.
Alternative Names
Yp1; CG2985; Vitellogenin-1; Vitellogenin I; Yolk protein 1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
21-439
Target Protein Sequence
PNGRMDNSVN QALKPSQWLS GSQLEAIPAL DDFTIERLEN MNLERGAELL QQVYHLSQIH HNVEPNYVPS GIQVYVPKPN GDKTVAPLNE MIQRLKQKQN FGEDEVTIIV TGLPQTSETV KKATRKLVQA YMQRYNLQQQ RQHGKNGNQD YQDQSNEQRK NQRTSSEEDY SEEVKNAKTQ SGDIIVIDLG SKLNTYERYA MLDIEKTGAK IGKWIVQMVN ELDMPFDTIH LIGQNVGAHV AGAAAQEFTR LTGHKLRRVT GLDPSKIVAK SKNTLTGLAR GDAEFVDAIH TSVYGMGTPI RSGDVDFYPN GPAAGVPGAS NVVEAAMRAT RYFAESVRPG NERSFPAVPA NSLQQYKQND GFGKRAYMGI DTAHDLEGDY ILQVNPKSPF GRNAPAQKQS SYHGVHQAWN TNQDSKDYQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Vitellogenin is the major yolk protein of eggs where it is used as a food source during embryogenesis.
Gene References into Functions
  1. cloned the Drosophila yp1 gene into an E. coli expression vector and showed that the yolk protein produced by E. coli is taken up into ovaries of both Drosophila melanogaster and the malaria mosquito Anopheles gambiae PMID: 12230547
Subcellular Location
Secreted.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Synthesized in the fat body and ovarian follicle cells and accumulate in the oocyte.
Database Links

KEGG: dme:Dmel_CG2985

STRING: 7227.FBpp0071354

UniGene: Dm.6740

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