Recombinant Drosophila melanogaster Voltage-dependent calcium channel type D subunit alpha-1 (Ca-alpha1D), partial

Code CSB-YP638759DLU
MSDS
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Source Yeast
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Code CSB-EP638759DLU
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Source E.coli
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Code CSB-EP638759DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP638759DLU
MSDS
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Source Baculovirus
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Code CSB-MP638759DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ca-alpha1D
Uniprot No.
Alternative Names
Ca-alpha1D; DroCa1; l(2)35Fa; CG4894Voltage-dependent calcium channel type D subunit alpha-1; DmCa1D
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Encodes a dihydropyridine- and diltiazem-sensitive current in larval body wall muscle. Vital for embryonic development.
Gene References into Functions
  1. Co-immunoprecipitation analysis supports association between Ma2/d and Amph, and indirectly with Msp300. Ma2/d, in association with Msp300 and Amph, mediates interactions between the SR and the nuclear membrane. PMID: 30093550
  2. maximum intraburst firing rates require axonal calcium influx through Dmca1D channels, likely to enhance sodium channel de-inactivation via a fast afterhyperpolarization through BK channel activation PMID: 28986465
  3. The Ca(2+) current in the embryonic muscle was completely eliminated in Dmca1D mutants, indicating that the Dmca1D-encoded Ca(2+) channel is the major mediator of inward currents in the body wall muscles throughout the embryonic and larval stages. PMID: 26004544
  4. The study indicated that alpha(2)delta proteins have functions that are independent of their roles in the biophysics and localization of Ca(2+) channels and that synaptic architecture depends on these functions. PMID: 19820706
  5. This is the first characterization of an alpha2delta-3 mutant in any organism and indicates a necessary role for alpha2delta-3 in presynaptic vesicle release and calcium channel expression at active zones. PMID: 18171920

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Calcium channel alpha-1 subunit (TC 1.A.1.11) family
Tissue Specificity
Expressed in the adult body, head and leg. Highly expressed in the embryonic nervous system.
Database Links

KEGG: dme:Dmel_CG4894

STRING: 7227.FBpp0089047

UniGene: Dm.4099

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