Recombinant Drosophila melanogaster WAS protein family homolog 1 (wash)

Code CSB-YP025972DLU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP025972DLU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP025972DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP025972DLU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP025972DLU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
wash
Uniprot No.
Alternative Names
wash; CG13176WASH complex subunit 1; Protein washout; WAS protein family homolog 1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-499
Target Protein Sequence
MEESPYLHSP YQVAIIATDL HHEDTIIQAA QSLDCLHKTI NSIFERIDAR LARNGSKVED INNRVKRAQA KIDALVGSKR AIQIFAPARF PASDVLAPLP ATFPQVAANP LMEQQVDQLP QGTYSSHSAA DQKPDDADIF FHVRGDREQE SPLVAERKIT NRTAGLGILP AGGVRSVPSL MRFNTNEFAY GEDLNAWKRS LPPQNARRVA SQSTQLTGEK QLAPAPHSLA HGTTKLATPA GDLRYNPAAL AAPAIDVPLD LPDLPGIAND LQYEPVEEQT PIAPSQQFGD LPELPDLGLE EQDIIVQAIA AQTHIPGPVR RKSVGQCPSP VTAAPPPPPP PPPPPPPPPP AQTSAIPSPP PFPTKGAVKP LSPSLATPLN MPQPPPATED PRSELMAAIR NAGGVHGGRL RSPAAAPLDV VDNSRSKAGG AVTGDLMADL HNKLMLRRKG ISGSQNPVEA TAGNPLMQQL SRVIPPPVQP RKGSKSSDEH SEDDEDGWN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Acts as a nucleation-promoting factor by activating the Arp2/3 complex to induce actin polymerization. Participates in both linear- and branched-actin networks. Functions in linear-filament (bundled F-actin) by acting downstream of Rho1 and regulating actin and microtubule organization during oogenesis. Nucleates actin in an Arp2/3-dependent manner and exhibits F-actin and microtubule bundling and cross-linking activity in the egg chamber. During embryogenesis, acts downstream of Rho1 to activate the Arp2/3 complex which is necessary for the developmental migration of tail hemocytes anteriorly along the ventral midline. Its function in hemocyte transmigration is independent of the WASH complex. May play a role in endosomal sorting; its assembly in the WASH complex may regulate its nucleation-promoting factor (NPF) activity.
Gene References into Functions
  1. Drosophila WASH has conserved functions in integrin receptor recycling and lysosome neutralization. PMID: 27884932
  2. reveal a novel role for Wash in modulating nucleus morphology and in the organization of both chromatin and non-chromatin nuclear sub-structures PMID: 25754639
  3. Developmental hemocyte migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott-Aldrich syndrome family protein. PMID: 25739458
  4. Results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures. PMID: 19633175
Protein Families
WASH1 family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1