Recombinant Drosophila melanogaster tRNA (guanine-N(7)-)-methyltransferase subunit wuho(wuho)

Code CSB-YP892937DLU
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Source Yeast
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Code CSB-EP892937DLU
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Source E.coli
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Code CSB-EP892937DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892937DLU
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Source Baculovirus
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Code CSB-MP892937DLU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names wuho
Uniprot No. Q9W415
Alternative Names wuho; CG15897; tRNA; guanine-N(7)-)-methyltransferase non-catalytic subunit wuho
Species Drosophila melanogaster (Fruit fly)
Expression Region 1-424
Target Protein Sequence MCTTISFAEP EIVLGHGRRV LFVNPDDLQI FKEIELPPDL GLKGHTSQSQ ESCTAAAAAS TATAASGQAP GGKEQQLANQ PEEGGTSASA SGLGCATSTS VQNVAYSPDG QLLAVTTSGK QKALLLYRSR PENARLLSAR PLARASSALR FCSDSSSILV TDKTGDCYQY DCVEVEAPPR LLLGHLSVVY DILWSEDQQH IITCDRDDKI RVTNYPATFD IHSYCLGHRE FVSGLALLTE QHIASASGDK TLRVWNYIQG KELLQHELPA PAVRLLVRQL EPEKVFQAAV LFYEHVDALG LYRLERSSDD TWSVTATQLV CAEAGSWSIS NFTLTSDRIY ITGAENERLS LRVYDIATGQ PASSGVPEGW LKMVLDGLGA NEEGAPPFIP EDLSVWFKKR FDNVSDYLER KKRRIEEQQQ QKCG
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit. Required during gametogenesis.
Gene References into Functions
  1. CG15897, here named wuho (wh) contains 5 WD40 repeats; has a critical function in cellular differentiation for germline cells during gametogenesis PMID: 16762337
Subcellular Location Nucleus. Note=Localized to the nuclei of nurse cells, follicle cells and oocytes at early stages, from germarium to stage 4 egg chambers. Also present in the nuclei of spermatocytes and in the apical cells of the testes.
Protein Families WD repeat TRM82 family
Tissue Specificity In testis, it is present at high level in hub cells, a niche for germline stem cells of testis.
Database Links

KEGG: dme:Dmel_CG15897

STRING: 7227.FBpp0070862

UniGene: Dm.31038

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