Recombinant ESX-1 secretion-associated protein EspB (espB)

Code CSB-YP835906MVZ
MSDS
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Source Yeast
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Code CSB-EP835906MVZ
MSDS
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Source E.coli
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Code CSB-EP835906MVZ-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835906MVZ
MSDS
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Source Baculovirus
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Code CSB-MP835906MVZ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
espB
Uniprot No.
Alternative Names
espB; mtb48; Rv3881c; MTV027.16cESX-1 secretion-associated protein EspB; Antigen MTB48
Species
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Expression Region
1-460
Target Protein Sequence
MTQSQTVTVD QQEILNRANE VEAPMADPPT DVPITPCELT AAKNAAQQLV LSADNMREYL AAGAKERQRL ATSLRNAAKA YGEVDEEAAT ALDNDGEGTV QAESAGAVGG DSSAELTDTP RVATAGEPNF MDLKEAARKL ETGDQGASLA HFADGWNTFN LTLQGDVKRF RGFDNWEGDA ATACEASLDQ QRQWILHMAK LSAAMAKQAQ YVAQLHVWAR REHPTYEDIV GLERLYAENP SARDQILPVY AEYQQRSEKV LTEYNNKAAL EPVNPPKPPP AIKIDPPPPP QEQGLIPGFL MPPSDGSGVT PGTGMPAAPM VPPTGSPGGG LPADTAAQLT SAGREAAALS GDVAVKAASL GGGGGGGVPS APLGSAIGGA ESVRPAGAGD IAGLGQGRAG GGAALGGGGM GMPMGAAHQG QGGAKSKGSQ QEDEALYTED RAWTEAVIGN RRRQDSKESK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for host-cell death and may support an EsxA-independent virulence function. Secreted processed form of EspB binds to phosphatidic acid and phosphatidylserine. Inhibits IFN-gamma-induced autophagy in murine macrophages.
Gene References into Functions
  1. EspB inhibits autophagosome formation in murine macrophages, at least in part by downregulating IFN-gamma receptor 1 expression. Overall, EspB should be considered a relevant factor in the pathogenesis of mycobacterial infections in humans. PMID: 25641595
  2. The structure of full-length EspB is presented. PMID: 26051906
Subcellular Location
Secreted.
Protein Families
EspB family
Database Links

KEGG: mtu:Rv3881c

STRING: 83332.Rv3881c

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