Product Details

Purity

>85% (SDS-PAGE)

Target Names

lepA

Uniprot No.

A1AE99

Alternative Names

lepA; Ecok1_24950; APECO1_3962Elongation factor 4; EF-4; EC 3.6.5.n1; Ribosomal back-translocase LepA

Species

Escherichia coli O1:K1 / APEC

Expression Region

1-599

Target Protein Sequence

MKNIRNFSII AHIDHGKSTL SDRIIQICGG LSDREMEAQV LDSMDLERER GITIKAQSVT LDYKASDGET YQLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAGQGVEA QTLANCYTAM EMDLEVVPVL NKIDLPAADP ERVAEEIEDI VGIDATDAVR CSAKTGVGVQ DVLERLVRDI PPPEGDPEGP LQALIIDSWF DNYLGVVSLI RIKNGTLRKG DKVKVMSTGQ TYNADRLGIF TPKQVDRTEL KCGEVGWLVC AIKDIHGAPV GDTLTLARNP AEKALPGFKK VKPQVYAGLF PVSSDDYEAF RDALGKLSLN DASLFYEPES SSALGFGFRC GFLGLLHMEI IQERLEREYD LDLITTAPTV VYEVETTSRE VIYVDSPSKL PAVNNIYELR EPIAECHMLL PQAYLGNVIT LCVEKRGVQT NMVYHGNQVA LTYEIPMAEV VLDFFDRLKS TSRGYASLDY NFKRFQASDM VRVDVLINGE RVDALALITH RGNSQNRGRE LVEKMKDLIP RQQFDIAIQA AIGTHIIARS TVKQLRKNVL AKCYGGDISR KKKLLQKQKE GKKRMKQIGN VELPQEAFLA ILHVGKDNK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

What affinity tag was used at which site? Does the difference of tag location affect activity of the protein?

The tag type will be determined during production process. We can try to express your desired tag first if you have special request for the tag, but as we can't guarantee, if it's failed, we will commumnicate with you and provide the protein with other tag.
The difference of tag location may affect activity of the protein, but the probability is small.

Could you express EF4 with an N-terminal hexa-histidine (His6) tag? For the formulation buffer, could you try 10mM Citric Acid 6% Sucrose in pH 6.0?

Yes, we can try your desired N-terminal 6xHis-tagged first.
For buffer, we can try 10mM Citric Acid in pH 6.0. Instead of 6% Sucrose, we normally add 6% Trehalose as protective agent.
But there is no guarantee that the protein will be stable under this buffer condition. If it is unstable, we will communicate with you in adavance and provide default Tris/PBS/HEPES based buffer.

Target Background

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.

Subcellular Location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, LepA subfamily

Database Links

KEGG: ecv:APECO1_3962

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Recombinant Escherichia coli O1:K1 / APEC Elongation factor 4 (lepA)

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Target Background

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