Recombinant Enterobacteria phage T4 ATP-dependent helicase 41 (41)

Code CSB-YP356281EDZ
MSDS
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Source Yeast
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Code CSB-EP356281EDZ
MSDS
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Source E.coli
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Code CSB-EP356281EDZ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP356281EDZ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP356281EDZ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
41
Uniprot No.
Alternative Names
41; ATP-dependent helicase 41; EC 3.6.4.-; Primase-helicase; Protein Gp41
Species
Enterobacteria phage T4 (Bacteriophage T4)
Expression Region
1-475
Target Protein Sequence
MVEIILSHLI FDQAYFSKVW PYMDSEYFES GPAKNTFKLI KSHVNEYHSV PSINALNVAL ENSSFTETEY SGVKTLISKL ADSPEDHSWL VKETEKYVQQ RAMFNATSKI IEIQTNAELP PEKRNKKMPD VGAIPDIMRQ ALSISFDSYV GHDWMDDYEA RWLSYMNKAR KVPFKLRILN KITKGGAETG TLNVLMAGVN VGKSLGLCSL AADYLQLGHN VLYISMEMAE EVCAKRIDAN MLDVSLDDID DGHISYAEYK GKMEKWREKS TLGRLIVKQY PTGGADANTF RSLLNELKLK KNFVPTIIIV DYLGICKSCR IRVYSENSYT TVKAIAEELR ALAVETETVL WTAAQVGKQA WDSSDVNMSD IAESAGLPAT ADFMLAVIET EELAAAEQQL IKQIKSRYGD KNKWNKFLMG VQKGNQKWVE IEQDSTPTEV NEVAGSQQIQ AEQNRYQRNE STRAQLDALA NELKF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ATP-dependent DNA helicase essential for viral DNA replication and recombination. The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis. Interaction with the primase allows the primase to initiate lagging strand synthesis and fully activates the helicase. Loaded by the helicase assembly factor on replication forks that begin at discrete replication origin sequences, as well as on forks that are created during recombination.
Gene References into Functions
  1. Data indicate that hexameric gp41 helicase binds weakly to the ss-dsDNA junction of a replication fork. PMID: 24062430
Database Links

KEGG: vg:1258700

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