Recombinant Enterobacteria phage T4 DNA polymerase processivity component (45)

Code CSB-YP361288EDZ
MSDS
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Source Yeast
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Code CSB-EP361288EDZ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP361288EDZ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP361288EDZ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
45
Uniprot No.
Alternative Names
45DNA polymerase clamp; DNA polymerase accessory protein 45; DNA polymerase sliding clamp; Gp45
Species
Enterobacteria phage T4 (Bacteriophage T4)
Expression Region
1-228
Target Protein Sequence
MKLSKDTTAL LKNFATINSG IMLKSGQFIM TRAVNGTTYA EANISDVIDF DVAIYDLNGF LGILSLVNDD AEISQSEDGN IKIADARSTI FWPAADPSTV VAPNKPIPFP VASAVTEIKA EDLQQLLRVS RGLQIDTIAI TVKEGKIVIN GFNKVEDSAL TRVKYSLTLG DYDGENTFNF IINMANMKMQ PGNYKLLLWA KGKQGAAKFE GEHANYVVAL EADSTHDF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sliding clamp that encircles the genomic DNA and links the DNA polymerase to the template to control the processivity of DNA synthesis. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Interaction with the sliding-clamp-loader opens the sliding clamp so that it can be loaded around the DNA template. During transcription, encircles the DNA and tethers host RNA polymerase (RNAP) to it.
Gene References into Functions
  1. The results suggest that the two domains of gp45 demonstrate an "inter-monomer" crosstalk that stabilizes the trimer. PMID: 27554844
  2. To form a productive holoenzyme complex, clamp loader protein gp44/62 is required for the loading of gp45, along with MgATP, and also for the subsequent binding of polymerase to the loaded clamp. PMID: 16800624
Protein Families
Tevenvirinae DNA polymerase processivity component family
Database Links

KEGG: vg:1258821

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