Recombinant Enterobacteria phage T4 Exonuclease subunit 1 (47)

Code CSB-YP366174EDZ
MSDS
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Source Yeast
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Code CSB-EP366174EDZ
MSDS
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Source E.coli
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Code CSB-EP366174EDZ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP366174EDZ
MSDS
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Source Baculovirus
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Code CSB-MP366174EDZ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
47
Uniprot No.
Alternative Names
47; Exonuclease subunit 1; EC 3.1.11.-; Gene product 47; gp47
Species
Enterobacteria phage T4 (Bacteriophage T4)
Expression Region
1-339
Target Protein Sequence
MKILNLGDWH LGVKADDEWI RGIQIDGIKQ AIEYSKKNGI TTWIQYGDIF DVRKAITHKT MEFAREIVQT LDDAGITLHT IVGNHDLHYK NVMHPNASTE LLAKYPNVKV YDKPTTVDFD GCLIDLIPWM CEENTGEILE HIKTSSASFC VGHWELNGFY FYKGMKSHGL EPDFLKTYKE VWSGHFHTIS EAANVRYIGT PWTLTAGDEN DPRGFWMFDT ETERTEFIPN NTTWHRRIHY PFKGKIDYKD FTNLSVRVIV TEVDKNLTKF ESELEKVVHS LRVVSKIDNS VESDDSEEVE VQSLQTLMEE YINAIPDITD SDREALIQYA NQLYVEATQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Exonuclease that plays a role in viral genome replication, DNA recombination, and host DNA degradation.
Gene References into Functions
  1. examine the kinetic and catalytic mechanism of ATP hydrolysis by Rad50 (gp46) in the presence and absence of Mre11 (gp47) and DNA PMID: 25137526
  2. Constructed an Mre11(gp46) mutant protein with the conserved histidine changed to serine. The mutant protein was found to be completely defective for nuclease activities, but retained the ability to bind the Rad50(gp47) subunit and double-stranded DNA. PMID: 23979587
Database Links

KEGG: vg:1258781

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