Recombinant Enterobacteria phage T7 Protein kinase (0.7)

Code CSB-YP360548EEB
MSDS
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Source Yeast
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Code CSB-EP360548EEB
MSDS
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Source E.coli
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Code CSB-EP360548EEB-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360548EEB
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP360548EEB
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
0.7
Uniprot No.
Alternative Names
0.7; Protein kinase 0.7; EC 2.7.11.1; Gene product 0.7; Gp0.7; Protein kinase gp0.7
Species
Enterobacteria phage T7 (Bacteriophage T7)
Expression Region
1-359
Target Protein Sequence
MNITDIMNAI DAIKALPICE LDKRQGMLID LLVEMVNSET CDGELTELNQ ALEHQDWWTT LKCLTADAGF KMLGNGHFSA AYSHPLLPNR VIKVGFKKED SGAAYTAFCR MYQGRPGIPN VYDVQRHAGC YTVVLDALKD CERFNNDAHY KYAEIASDII DCNSDEHDEL TGWDGEFVET CKLIRKFFEG IASFDMHSGN IMFSNGDVPY ITDPVSFSQK KDGGAFSIDP EELIKEVEEV ARQKEIDRAK ARKERHEGRL EARRFKRRNR KARKAHKAKR ERMLAAWRWA ERQERRNHEV AVDVLGRTNN AMLWVNMFSG DFKALEERIA LHWRNADRMA IANGLTLNID KQLDAMLMG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine/threonine kinase that modulates host metabolism to favor virus replication cycle. Participates together with gene 2 protein (gp2) in the host transcription shutoff. Phosphorylates host RNA polymerase subunit RpoC and to a lesser extent subunit RpoB. This modification seems to increase rho-dependent transcription termination, which may help to switch from host to viral RNA polymerase transcription during phage development. Phosphorylates host components of the RNA degradosome rne and RhlB, leading to stabilization of mRNAs synthesized by T7 RNA polymerase. Phosphorylates host dsRNA specific processing enzyme RNase III/rnc, leading to enhance activity of the ribonuclease that may increase maturation of T7 late transcripts possessing multiple Rnase III processing sites. Phosphorylates several components of the host translational machinery including initiation factors InfA, InfB, and InfC, ribosomal subunit RpsA and RpsF, as well as elongation factor G/FusA. These phosphorylations may enhance T7 late protein production.
Database Links

KEGG: vg:1261070

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