Recombinant Escherichia coli DNA helicase II(uvrD)

Code CSB-EP360936ENV
Size US$2632
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360936ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) uvrD.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360936ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) uvrD.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names uvrD
Uniprot No. P03018
Research Area Others
Alternative Names uvrD; mutU; pdeB; rad; recL; b3813; JW3786; DNA helicase II; EC 3.6.4.12
Species Escherichia coli (strain K12)
Source E.coli
Expression Region 1-720aa
Target Protein Sequence MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 82.0 kDa
Protein Length Full Length
Tag Info Tag-Free
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function A helicase with DNA-dependent ATPase activity
Gene References into Functions
  1. Large domain movements upon UvrD dimerization and helicase activation has been reported. PMID: 29087333
  2. Results show that UvrD monomer translocation rate depends on the single-stranded DNA base composition, even in the absence of any predicted basepairing within the nucleic acid. Translocation is faster on single-stranded DNA-containing pyrimidines and slower on ssDNA-containing purines PMID: 28978435
  3. these data reveal that UvrD exhibits two distinct types of unwinding activity regulated by its stoichiometry. Furthermore, two UvrD conformational states, termed "closed" and "open," correlate with movement toward or away from the DNA fork. PMID: 25883359
  4. The unwinding processivity is increased in the UvrD303 mutant. PMID: 24798324
  5. UvrD binds RNA polymerase during transcription elongation, using its helicase/translocase activity, forces RNA polymerase to slide backward along DNA; UvrD exposes DNA lesions, allowing nucleotide excision repair enzymes to gain access to sites of damage PMID: 24402227
  6. These results suggest that UvrD pauses more frequently during translocation at low ATP but with little futile ATP hydrolysis. PMID: 22342931
  7. The anti-thymineless death role of UvrD. PMID: 21705756
  8. crystal structure of an apo form of UvrD in which the 2B sub-domain is in an "open" state that differs by 160 degrees rotation of the 2B sub-domain PMID: 21704638
  9. Although a 3'-ssDNA partial duplex provides a high-affinity site for a uvrD monomer, the study shows that a monomer also binds with specificity to DNA junctions possessing a 5'-ssDNA flanking region and can initiate translocation from this site. PMID: 20877334
  10. UvrD has a critical role in the processing of DNA-protein cross-links PMID: 20444702
  11. UvrD monomers translocate in discrete steps with an average kinetic step-size, m=3.68 nt step(-1), a translocation rate constant, kt=51.3 steps s(-1), with a processivity corresponding to an average translocation distance of 2400 nt before dissociation PMID: 15561144
  12. mutational analysis of a thermostable UvrD helicase PMID: 15955821
  13. acts by removing RecA or a RecA-made structure, allowing replication fork reversal PMID: 16135232
  14. UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites. PMID: 17020578
  15. Role of UvrD on RecET-mediated illegitimate recombination. PMID: 17038801
  16. Our results suggest that macroscopic rate of UvrD monomer translocation is not limited by each ATPase cycle but by a slow step (pause) in each translocation cycle that occurs after four to five rapid 1 nt translocation steps. PMID: 17499041
  17. Results show that the anti-RecA action of UvrD at blocked forks reflects two different activities of this enzyme. PMID: 17641684
  18. The multiform patterns of unwinding bursts and the distributions of the off-times favour the mechanism that UvrD unwinds DNA as a dimer. PMID: 19008855

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Protein Families Helicase family, UvrD subfamily
Database Links

KEGG: ecj:JW3786

STRING: 316385.ECDH10B_4005

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