Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

uvrD

Uniprot No.

P03018

Research Area

Others

Alternative Names

uvrD; mutU; pdeB; rad; recL; b3813; JW3786; DNA helicase II; EC 3.6.4.12

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-720aa

Target Protein Sequence

MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

82.0 kDa

Protein Length

Full Length

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The gene fragment corresponding to the 1-720aa of the E.coli uvrD protein was cloned into an expression vector. The expression vector was transformed into the E.coli for expression. The generated product was purified and separated to obtain the recombinant E.coli uvrD protein. Its purity is higher than 85% measured by SDS-PAGE. This recombinant uvrD protein showed an apparent molecular weight of about 90 kDa under SDS-PAGE condition.

uvrD is a gene encoding a protein named DNA helicase II and belongs helicase family and UvrD subfamily. Helicases are a class of enzymes thought to be vital to all organisms. Their main function is to unpack an organism's genes.uvrD is a helicase with DNA-dependent ATPase activity and has a critical role in the processing of DNA-protein cross-links. It is involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA.

Target Background

Function

A helicase with DNA-dependent ATPase activity. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand. Initiates unwinding more efficiently from a nicked substrate than ds duplex DNA. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair, and probably also in repair of alkylated DNA.

Gene References into Functions

Large domain movements upon UvrD dimerization and helicase activation has been reported. PMID: 29087333
Results show that UvrD monomer translocation rate depends on the single-stranded DNA base composition, even in the absence of any predicted basepairing within the nucleic acid. Translocation is faster on single-stranded DNA-containing pyrimidines and slower on ssDNA-containing purines PMID: 28978435
these data reveal that UvrD exhibits two distinct types of unwinding activity regulated by its stoichiometry. Furthermore, two UvrD conformational states, termed "closed" and "open," correlate with movement toward or away from the DNA fork. PMID: 25883359
The unwinding processivity is increased in the UvrD303 mutant. PMID: 24798324
UvrD binds RNA polymerase during transcription elongation, using its helicase/translocase activity, forces RNA polymerase to slide backward along DNA; UvrD exposes DNA lesions, allowing nucleotide excision repair enzymes to gain access to sites of damage PMID: 24402227
These results suggest that UvrD pauses more frequently during translocation at low ATP but with little futile ATP hydrolysis. PMID: 22342931
The anti-thymineless death role of UvrD. PMID: 21705756
crystal structure of an apo form of UvrD in which the 2B sub-domain is in an "open" state that differs by 160 degrees rotation of the 2B sub-domain PMID: 21704638
Although a 3'-ssDNA partial duplex provides a high-affinity site for a uvrD monomer, the study shows that a monomer also binds with specificity to DNA junctions possessing a 5'-ssDNA flanking region and can initiate translocation from this site. PMID: 20877334
UvrD has a critical role in the processing of DNA-protein cross-links PMID: 20444702
UvrD monomers translocate in discrete steps with an average kinetic step-size, m=3.68 nt step(-1), a translocation rate constant, kt=51.3 steps s(-1), with a processivity corresponding to an average translocation distance of 2400 nt before dissociation PMID: 15561144
mutational analysis of a thermostable UvrD helicase PMID: 15955821
acts by removing RecA or a RecA-made structure, allowing replication fork reversal PMID: 16135232
UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites. PMID: 17020578
Role of UvrD on RecET-mediated illegitimate recombination. PMID: 17038801
Our results suggest that macroscopic rate of UvrD monomer translocation is not limited by each ATPase cycle but by a slow step (pause) in each translocation cycle that occurs after four to five rapid 1 nt translocation steps. PMID: 17499041
Results show that the anti-RecA action of UvrD at blocked forks reflects two different activities of this enzyme. PMID: 17641684
The multiform patterns of unwinding bursts and the distributions of the off-times favour the mechanism that UvrD unwinds DNA as a dimer. PMID: 19008855

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Protein Families

Helicase family, UvrD subfamily

Database Links

KEGG: ecj:JW3786

STRING: 316385.ECDH10B_4005

Code	CSB-EP360936ENV
Abbreviation	Recombinant E.coli uvrD protein
MSDS	MSDS Datasheet
Size	US$472
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360936ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) uvrD. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360936ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) uvrD.
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Recombinant Escherichia coli DNA helicase II (uvrD)

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