Product Details

Purity

>85% (SDS-PAGE)

Target Names

rpiA

Uniprot No.

B1LDB5

Alternative Names

rpiA; EcSMS35_3047; Ribose-5-phosphate isomerase A; EC 5.3.1.6; Phosphoriboisomerase A; PRI

Species

Escherichia coli (strain SMS-3-5 / SECEC)

Expression Region

1-219

Target Protein Sequence

MTQDELKKAV GWAALQYVQP GTIVGVGTGS TAAHFIDALG TMKGQIEGAV SSSDASTEKL KSLGIHVFDL NEVDSLGIYV DGADEINGHM QMIKGGGAAL TREKIIASVA EKFICIADAS KQVDILGKFP LPVEVIPMAR SAVARQLVKL GGRPEYRQGV VTDNGNVILD VHGMEILDPI AMENAINAIP GVVTVGLFAN RGADVALIGT PDGVKTIVK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I am looking to buy the protein RPI-A with an N-terminal HIS-tag. As I work with highly sensitive aptamer tools, I need to know the exact sequence before I buy. Is it only the protein plus 6xHIS, or is there a linker contained as well? If so, do you know if it impacts the structure?

Thanks for your inquiry.
Recombinant Escherichia coli Ribose-5-phosphate isomerase A(rpiA)
Express Region: 1-219aa; Full length protein.
Tag information:We are not sure you need C terminal 6*his tag or N terminal 6*his tag. We will try to link to the tag you want in prior, if we can't provide it, we could provide other tags. ( The success rate is 95.25%）
Sequence:

MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKGQIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTREKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGVVTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIVK

If you need 6*his, there will be no linker. If you need the fusion protein with other tags, there will be linkers. Usually it doesn't impact the structurer.
Shipping format: The default delivery form is liquid form. If you have demand for lyophilized form, please remark this requirement when placing order.
Optional service: Do you want aseptic processing and/or endotoxin removal for this protein (recommended for cell culture or in vivo use)? With our endotoxin removal service we guarantee the endotoxin level to be less than 0.1ng/ug (1EU ug). Both aseptic processing and endotoxin removal services are free of charge however they must be requested when the order is placed.
Western Blot service: We could offer Western Blot service for tag antibody free of charge. If you have demand, please remark this information when placing the order.
Shipping condition: Our proteins are routinely shipped on ice packs.

Target Background

Function

Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.

Protein Families

Ribose 5-phosphate isomerase family

Database Links

KEGG: ecm:EcSMS35_3047

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Recombinant Escherichia coli Ribose-5-phosphate isomerase A (rpiA)

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