Recombinant Escherichia coli ATP-dependent RNA helicase rhlE (rhlE)

Code CSB-YP335156ENV
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP335156ENV
MSDS
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Source E.coli
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Code CSB-EP335156ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335156ENV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP335156ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rhlE
Uniprot No.
Alternative Names
rhlE; b0797; JW0781; ATP-dependent RNA helicase RhlE; EC 3.6.4.13
Species
Escherichia coli (strain K12)
Expression Region
1-454
Target Protein Sequence
MSFDSLGLSP DILRAVAEQG YREPTPIQQQ AIPAVLEGRD LMASAQTGTG KTAGFTLPLL QHLITRQPHA KGRRPVRALI LTPTRELAAQ IGENVRDYSK YLNIRSLVVF GGVSINPQMM KLRGGVDVLV ATPGRLLDLE HQNAVKLDQV EILVLDEADR MLDMGFIHDI RRVLTKLPAK RQNLLFSATF SDDIKALAEK LLHNPLEIEV ARRNTASDQV TQHVHFVDKK RKRELLSHMI GKGNWQQVLV FTRTKHGANH LAEQLNKDGI RSAAIHGNKS QGARTRALAD FKSGDIRVLV ATDIAARGLD IEELPHVVNY ELPNVPEDYV HRIGRTGRAA ATGEALSLVC VDEHKLLRDI EKLLKKEIPR IAIPGYEPDP SIKAEPIQNG RQQRGGGGRG QGGGRGQQQP RRGEGGAKSA SAKPAEKPSR RLGDAKPAGE QQRRRRPRKP AAAQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DEAD-box RNA helicase involved in ribosome assembly. Has RNA-dependent ATPase activity and unwinds double-stranded RNA. May play a role in the interconversion of ribosomal RNA-folding intermediates that are further processed by DeaD or SrmB during ribosome maturation.
Gene References into Functions
  1. presented in vitro evidence that SrmB, RhlE and CsdA can bind Rnase E at a site that is different from the rhlB binding site; RhlE can replace the function of RhlB in vitro PMID: 15554979
  2. RhlE is a novel ribosome assembly factor, which plays a role in the interconversion of ribosomal RNA-folding intermediates that are further processed by DeaD or SrmB during ribosome maturation. PMID: 18083833
Subcellular Location
Cytoplasm. Note=Ribosome-associated. Can either bind to the intact 70S ribosome or to individual ribosomal subunits.
Protein Families
DEAD box helicase family, RhlE subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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