Recombinant Escherichia coli Beta-lactamase(ampC)

In Stock
Code CSB-EP360448ENV
Size US$388
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Alternative Names
ampC; ampA; b4150; JW4111Beta-lactamase; EC; Cephalosporinase
Escherichia coli (strain K12)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
52.5 kDa
Protein Length
Mature full length protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Tris-based buffer,50% glycerol
and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Just like other recombinant proteins, the production of this recombinant E.coli ampC protein began with appropriate cDNA and PCR methods, and then the ampC expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant E.coli ampC protein. N-terminal 6xHis-SUMO tag was used in the process. And we finally get the protein of interest with purity of 85%+.

ampC (also called ampA) is a gene providing an instruction of making a protein named beta-lactamase (also known as cephalosporinase) in Escherichia coli (E.coli). This protein belongs to Class-C beta-lactamase family, which are enzymes produced by bacteria, E.coli and Klebsiella. The protein encoded by ampC hydrolyzes the beta-lactam bond in susceptible beta-lactam antibiotics and takes part in antibiotic catabolic process and the process of response to antibiotic.

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Target Background

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
Gene References into Functions
  1. These findings demonstrate that animal-based food products in HCMC represent a major reservoir of ESBL/pAmpC-producing E. coli. PMID: 26989692
  2. Recently, high-level AmpC production has also been seen in new species such as Escherichia coli via plasmid acquisition. PMID: 25643271
  3. the majority of the strains of E. coli with AmpC-susceptibility pattern are pAmpC positive, although the association of extended-spectrum beta-lactamases (ESBL) and pAmpC is unusual. PMID: 24372614
  4. A high prevalence of birds carries extended spectrumBL(ESBL)/AmpC-producing E. coli at Dutch broiler farms and a high prevalence of ESBL/AmpC-producing E. coli in farmers. PMID: 22949623
  5. Extended-spectrum beta-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors. PMID: 23243181
  6. Cefoxitin resistance in E. coli N99-0001 was due to overexpression of ampC caused by an increase in promoter strength. PMID: 15761065
  7. determined structure of ampC K67R mutant by X-ray crystallography in apo & transition state-analog complexed forms; results suggest arginine side chain is unable to play the same catalytic role as Lys67 in either the acylation or deacylation reactions PMID: 19241376

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Subcellular Location
Protein Families
Class-C beta-lactamase family
Database Links
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