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Recombinant Escherichia coli Beta-lactamase (ampC)

Recombinant Escherichia coli Beta-lactamase (ampC)

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Code	CSB-EP360448ENV
Abbreviation	Recombinant E.coli ampC protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ampC

Uniprot No.

P00811

Research Area

Microbiology

Alternative Names

ampC; ampA; b4150; JW4111Beta-lactamase; EC 3.5.2.6; Cephalosporinase

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

20-377aa

Target Protein Sequence

APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

52.5 kDa

Protein Length

Mature full length protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Just like other recombinant proteins, the production of this recombinant E.coli ampC protein began with appropriate cDNA and PCR methods, and then the ampC expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant E.coli ampC protein. N-terminal 6xHis-SUMO tag was used in the process. And we finally get the protein of interest with purity of 85%+.

ampC (also called ampA) is a gene providing an instruction of making a protein named beta-lactamase (also known as cephalosporinase) in Escherichia coli (E.coli). This protein belongs to Class-C beta-lactamase family, which are enzymes produced by bacteria, E.coli and Klebsiella. The protein encoded by ampC hydrolyzes the beta-lactam bond in susceptible beta-lactam antibiotics and takes part in antibiotic catabolic process and the process of response to antibiotic.

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Target Background

Function

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Gene References into Functions

These findings demonstrate that animal-based food products in HCMC represent a major reservoir of ESBL/pAmpC-producing E. coli. PMID: 26989692
Recently, high-level AmpC production has also been seen in new species such as Escherichia coli via plasmid acquisition. PMID: 25643271
the majority of the strains of E. coli with AmpC-susceptibility pattern are pAmpC positive, although the association of extended-spectrum beta-lactamases (ESBL) and pAmpC is unusual. PMID: 24372614
A high prevalence of birds carries extended spectrumBL(ESBL)/AmpC-producing E. coli at Dutch broiler farms and a high prevalence of ESBL/AmpC-producing E. coli in farmers. PMID: 22949623
Extended-spectrum beta-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors. PMID: 23243181
Cefoxitin resistance in E. coli N99-0001 was due to overexpression of ampC caused by an increase in promoter strength. PMID: 15761065
determined structure of ampC K67R mutant by X-ray crystallography in apo & transition state-analog complexed forms; results suggest arginine side chain is unable to play the same catalytic role as Lys67 in either the acylation or deacylation reactions PMID: 19241376