Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Microbiology
Alternative Names
ampC; ampA; b4150; JW4111Beta-lactamase; EC 3.5.2.6; Cephalosporinase
Species
Escherichia coli (strain K12)
Expression Region
20-377aa
Target Protein Sequence
APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Mature full length protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Just like other recombinant proteins, the production of this recombinant E.coli ampC protein began with appropriate cDNA and PCR methods, and then the ampC expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant E.coli ampC protein. N-terminal 6xHis-SUMO tag was used in the process. And we finally get the protein of interest with purity of 85%+.
ampC (also called ampA) is a gene providing an instruction of making a protein named beta-lactamase (also known as cephalosporinase) in Escherichia coli (E.coli). This protein belongs to Class-C beta-lactamase family, which are enzymes produced by bacteria, E.coli and Klebsiella. The protein encoded by ampC hydrolyzes the beta-lactam bond in susceptible beta-lactam antibiotics and takes part in antibiotic catabolic process and the process of response to antibiotic.
