ampC Antibody

Code CSB-PA360448XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) ampC Polyclonal antibody
Uniprot No.
Target Names
ampC
Alternative Names
ampC antibody; ampA antibody; b4150 antibody; JW4111Beta-lactamase antibody; EC 3.5.2.6 antibody; Cephalosporinase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) ampC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
Gene References into Functions
  1. These findings demonstrate that animal-based food products in HCMC represent a major reservoir of ESBL/pAmpC-producing E. coli. PMID: 26989692
  2. Recently, high-level AmpC production has also been seen in new species such as Escherichia coli via plasmid acquisition. PMID: 25643271
  3. the majority of the strains of E. coli with AmpC-susceptibility pattern are pAmpC positive, although the association of extended-spectrum beta-lactamases (ESBL) and pAmpC is unusual. PMID: 24372614
  4. A high prevalence of birds carries extended spectrumBL(ESBL)/AmpC-producing E. coli at Dutch broiler farms and a high prevalence of ESBL/AmpC-producing E. coli in farmers. PMID: 22949623
  5. Extended-spectrum beta-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors. PMID: 23243181
  6. Cefoxitin resistance in E. coli N99-0001 was due to overexpression of ampC caused by an increase in promoter strength. PMID: 15761065
  7. determined structure of ampC K67R mutant by X-ray crystallography in apo & transition state-analog complexed forms; results suggest arginine side chain is unable to play the same catalytic role as Lys67 in either the acylation or deacylation reactions PMID: 19241376

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Subcellular Location
Periplasm.
Protein Families
Class-C beta-lactamase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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