Recombinant Escherichia coli DNA polymerase II (polB)

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Code CSB-EP321908ENV
Size $256
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Greater than 90% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Alternative Names
(Pol II)
Escherichia coli (strain K12)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
97.4 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Achieving the recombinant Escherichia coli (strain K12) polB protein production entails controlling the expression of the desired gene in e.coli cells. The sequence (2-783aa) of foreign DNA is integrated into an expression vector and then transferred to e.coli cells. Selected positive cells are cultured to induce the expression of the desired protein. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. The recombinant Escherichia coli (strain K12) polB protein is purified through affinity purification, exhibiting a purity greater than 90%, as determined by SDS-PAGE.

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Target Background

Thought to be involved in DNA repair and/or mutagenesis. Its processivity is enhanced by the beta sliding clamp (dnaN) and clamp loader.
Gene References into Functions
  1. polB gene expression is controlled by SOS and RpoS regulons. PMID: 28468910
  2. Polymerases II, IV and V may bypass trans-4-hydroxy-2-nonenal modified bases by translesion synthesis and restore DNA synthesis beyond the arrested site, although with different efficiencies. PMID: 22001238
  3. Data report that 15% of stress-induced mutations associated with DNA double-strand break repair in carbon-starved Escherichia coli requires high-fidelity Pol II, and that this component also requires RpoS. PMID: 20639336
  4. transcription by single Pol II complexes allows survival of the original H3/H4 histones, while multiple, closely spaced complexes of transcribing Pol II can induce displacement of all core histones along the gene PMID: 20534568
  5. Compared to the replicative B family polymerases, DNA Pol II has subtle amino acid changes remote from the active site that allow it to replicate normal DNA with high efficiency yet conduct translesion synthesis when needed. PMID: 20064374
  6. The results fit a model in which Pol II acts as proofreader for HE-produced misinsertion errors. PMID: 16164549
  7. These data suggest that DNA polymerase II is responsible for the in vivo mutagenic bypass of these lesions in wild-type E. coli. PMID: 16257273
  8. elevated expression of Pol-II leads to an approximately 100-fold increase in spontaneous mutagenesis in a manner that is independent of SOS, umuDC, dinB, recA, uvrA and mutS functions PMID: 17586534
  9. Data show that an important role is described for DNA Pol II in preventing the mutations and reveal that Pol II is a major editor of Pol III mediated replication errors. PMID: 18156258

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Protein Families
DNA polymerase type-B family
Database Links

KEGG: ecj:JW0059

STRING: 316407.85674307

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