Product Details

Purity

>85% (SDS-PAGE)

Target Names

ftsK

Uniprot No.

P46889

Alternative Names

ftsK; b0890; JW0873; DNA translocase FtsK

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif. Stoppage of translocation is accompanied by a reduction in ATPase activity. Also stimulates topoisomerase 4 activity. Required for the targeting of FtsQ, FtsL and FtsI to the septum.

Gene References into Functions

FtsKgamma interacts with the C-terminal domain of XerD, above a cleft where XerC is thought to bind. We present a model for activation of recombination based on structural data. PMID: 27708355
We found that whatever the growth conditions, sister copies of the V. cholerae chr1 dif region remain together at mid-cell until the onset of constriction, which permits their processing by FtsK and the activation of dif-recombination. PMID: 28358835
FtsK translocase has a role in assembly, translocation, and activation of XerCD-dif recombination PMID: 26324908
An average of 7 FtsK hexamers per cell are present at midcell, with the N-terminal domain being able to hexamerize independently of the translocase. PMID: 24302254
Kinetic analyses showed that the presence of the FtsK gamma domain increases the rate of XerCD-dif synapse assembly and increases its stability. PMID: 24214995
Data indicate that the arrival of the FtsK translocase at an initial synaptic complex, forming a transient activated complex in which XerD exchanged a pair of strands to form an Holliday junctions (HJ) intermediate for catalysis by XerC. PMID: 24101525
FtsK segregates the terminus region of sister chromosomes whether they are monomeric or dimeric and does so in an accurate and ordered manner. PMID: 23781109
Multiple regions in FtsK protein are required for in cell division. PMID: 21091498
The properties in vivo and in vitro of FtsK and its relatives are discussed in relation to the biological functions of these remarkable enzymes. PMID: 20298190
FtsK transports DNA before membrane fusion, at a time when there is still an opening in the constricted septum. PMID: 20033058
FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. PMID: 19854947
Data describe the in vivo effects of deletions of FtsK(L) and/or of FtsK(C), of swaps of these domains with their Haemophilus influenzae counterparts and of a point mutation that inactivates the walker A motif of FtsK(C). PMID: 15522074
directly observed the movement of purified FtsK on single DNA molecules; results imply that FtsK is a bidirectional motor that changes direction in response to short, asymmetric directing DNA sequences PMID: 15681387
FtsK induces approximately 0.07 supercoils per DNA helical pitch traveled PMID: 15821742
translocation by FtsK occurs mostly on DNA belonging to a specifically organized domain of the chromosome, when physical links between either dimeric or still intercatenated chromosomes force this DNA to run across the septum at division PMID: 15916604
Results suggest that the N-terminal domain of FtsK is normally involved in stability of the division protein machine and shares functional overlap with FtsQ, FtsB, FtsA, ZipA and FtsN. PMID: 16194242
FtsK translocase is a powerful motor that is able to displace a triplex-forming oligo from a DNA substrate PMID: 16301526
A 62-amino-acid fragment of FtsK(C) interacts directly with the XerD C-terminus in order to stimulate the cleavage by XerD of BSN, a dif-DNA suicide substrate containing a nick in the 'bottom' strand. PMID: 16553881
Study provides evidence that 8-base-pair asymmetric sequences (KOPS) promote FtsK loading on DNA and that translocation is oriented at this step. PMID: 17041597
Study uses in vitro single-molecule and ensemble methods to unveil a mechanism of action in which the translocation and sequence-recognition activities are performed by different domains in FtsK. PMID: 17041598
controls recombination provoked by an extra Ter site in the chromosome terminus PMID: 17376083
Septum localization and concentration of FtsK co-ordinate its various roles in chromosome segregation and cell division. PMID: 18363794
FtsK interacts with the cell division partner proteins through different functional domians. PMID: 18759781
FtsK mediates a reciprocal control between processing of the replichore polarity junction and cell division. PMID: 19057667
Failure to recognize KOPS during FtsK translocation has only modest consequences for fitness of wild-type cells. PMID: 19170870
The structure and composition of DNA interferes with Xer recombination activation by FtsK. PMID: 19246541

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein. Note=Located at the septum. Colocalizes with FtsZ. ZipA, FtsZ and FtsA, but not FtsI and FtsQ are required to target it to the septum.

Protein Families

FtsK/SpoIIIE/SftA family

Database Links

KEGG: ecj:JW0873

STRING: 316385.ECDH10B_0960

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Recombinant Escherichia coli DNA translocase FtsK (ftsK), partial

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