Name: Recombinant Escherichia coli Exopolyphosphatase (ppx)
Brand: CUSABIO
SKU: CSB-EP365319ENV

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ppx

Uniprot No.

P0AFL6

Research Area

Microbiology

Alternative Names

ppx; b2502; JW2487; Exopolyphosphatase; ExopolyPase; EC 3.6.1.11; Metaphosphatase

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

2-513aa

Target Protein Sequence

PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

74.0kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The DNA fragment encoding the 2-513aa of the E.coli ppx protein was fused with N-terminal 6xHis-SUMO tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant E.coli ppx protein. The purity of this recombinant ppx protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant ppx protein showed a band on the gel with a molecular weight of approximately 68-91 kDa.

ppx is a gene encoding a protein named Exopolyphosphatase and belongs GppA/Ppx family. Exopolyphosphatase (PPX),a phosphatase enzyme, was discovered by the lab of Nobel laureate Arthur Kornberg in 1993.The structure of PPX is characterized by the actin-like ATPase domain. This protein catalyzes and releases orthophosphate processively from the ends of the polyP chain which is a polymer composed of a few to hundreds of phosphate residues supporting cell survival in the stationary phase. This enzyme plays important role in the bacterial stringent response.

Customer Reviews and Q&A

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■ Q&A

Regarding CSB-EP365319ENV (all expression systems) we want to confirm that Exopolyphosphatase can be used to degrade polyphosphates in vitro? If yes, What are the buffer conditions needed?

Thanks for your inquiry. We have successfully developed this EP protein, the default buffer is 20 mM Tris-HCl, 0.5M NaCl, pH 8.0, 50% glycerol.
There is also some other customer requests a desired buffer of 20 mM HEPES, pH 7.4, 10 % glycerol before.
Regarding using Exopolyphosphatase to degrade polyphosphates in vitro, we think the only requirement for the buffer is that it should not contain any phosphate.
Do you have any other special requirement for the buffer ? If you want to supply this protein is a special buffer, pls let us know in advance.

Target Background

Function

Degradation of inorganic polyphosphates (polyP). Releases orthophosphate processively from the ends of the polyP chain. Has a strong preference for long-chain polyphosphates and has only weak affinity for smaller size polyP of about 15 residues.

Gene References into Functions

H(378) is a fundamental gatekeeper for the recognition of long chain polyphosphate PMID: 27424154

Subcellular Location

Cell membrane; Peripheral membrane protein.

Protein Families

GppA/Ppx family

Database Links

KEGG: ecj:JW2487

STRING: 316385.ECDH10B_2668

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