Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

gadA

Uniprot No.

P69908

Research Area

Others

Alternative Names

gadA; gadS; b3517; JW3485Glutamate decarboxylase alpha; GAD-alpha; EC 4.1.1.15

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-466aa

Target Protein Sequence

MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

56.7kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Escherichia coli (strain K12) gadA covers amino acids 1-466. The theoretical molecular weight of the gadA protein is 56.7 kDa. The gadA protein was expressed in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of gadA, which enables a simple process of detecting and purifying the gadA recombinant protein in the following steps.

The Escherichia coli glutamate decarboxylase alpha (GadA) plays a crucial role in the bacterial acid stress response. GadA is part of the glutamate-dependent acid resistance (GDAR) system in E. coli, which helps the bacteria survive in acidic environments, such as the stomach. The enzyme catalyzes the decarboxylation of L-glutamate to form γ-aminobutyric acid (GABA) and carbon dioxide. This conversion contributes to the regulation of intracellular pH, acting as a buffer against acid stress. The GDAR system is essential for E. coli to pass through the acidic conditions of the stomach during host infection. Understanding the function of GadA is important not only for bacterial physiology but also for potential applications in biotechnology and food preservation.

Target Background

Function

Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.

Protein Families

Group II decarboxylase family

Database Links

KEGG: ecj:JW3485

STRING: 316385.ECDH10B_3694

Code	CSB-EP302526ENV
Abbreviation	Recombinant E.coli gadA protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli Glutamate decarboxylase alpha (gadA)

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