Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
gadA; gadS; b3517; JW3485Glutamate decarboxylase alpha; GAD-alpha; EC 4.1.1.15
Species
Escherichia coli (strain K12)
Expression Region
1-466aa
Target Protein Sequence
MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Escherichia coli (strain K12) gadA covers amino acids 1-466. The theoretical molecular weight of the gadA protein is 56.7 kDa. The gadA protein was expressed in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of gadA, which enables a simple process of detecting and purifying the gadA recombinant protein in the following steps.
The Escherichia coli glutamate decarboxylase alpha (GadA) plays a crucial role in the bacterial acid stress response. GadA is part of the glutamate-dependent acid resistance (GDAR) system in E. coli, which helps the bacteria survive in acidic environments, such as the stomach. The enzyme catalyzes the decarboxylation of L-glutamate to form γ-aminobutyric acid (GABA) and carbon dioxide. This conversion contributes to the regulation of intracellular pH, acting as a buffer against acid stress. The GDAR system is essential for E. coli to pass through the acidic conditions of the stomach during host infection. Understanding the function of GadA is important not only for bacterial physiology but also for potential applications in biotechnology and food preservation.
