Recombinant Escherichia coli Murein hydrolase activator NlpD(nlpD)

In Stock
Code CSB-EP359913ENV
Size US$2466
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details


This recombinant E.coli K12 nlpD protein is an E.coli-expressed protein (Full Length of Mature Protein). At the theoretical level, the steps needed for obtaining a recombinant protein are pretty straightforward. Obtain the nlpD gene, clone it in an expression vector, transform it into the host of choice, induce it, and then, the protein is ready for purification and characterization. In practice, however, dozens of things can go wrong. Poor growth of the host, inclusion body formation, protein inactivity, and even not obtaining any protein at all are some of the problems often found down the pipeline. So we choose the most suitable strain and vector for this recombinant nlpD protein expression and adopt the most optimized expression condition and purification method. Finally, mild condition and subsequent cumbersome protein-refolding procedures ensure this nlpD protein is soluble.

nlpD is a protein encoding gene that provides an instruction in making a protein named murein hydrolase activator NlpD. The protein encoded by this gene belongs to E.coli NlpD/Haemophilus LppB family. It is similar in sequence to the LppB lipoprotein outer membrane antigen of Haemophilus somnus in Escherichia coli. This protein has metalloendopeptidase activity and is involved in multiple biological processes, including cell cycle, positive regulation of hydrolase activity, response to drug and septum digestion after cytokinesis.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names nlpD
Uniprot No. P0ADA3
Research Area Microbiology
Alternative Names
nlpD; b2742; JW2712Murein hydrolase activator NlpD
Species Escherichia coli (strain K12)
Source E.coli
Expression Region 26-379aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 53.5kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
Gene References into Functions
  1. Structure-function analysis revealed that the outer membrane localization of NlpD is critical for regulating its amidase activation activity. PMID: 28708841
  2. The authors report that AmiB and EnvC are recruited to division sites independently of one another. The same is also true for AmiC and NlpD. PMID: 21764913
Subcellular Location Cell inner membrane; Lipid-anchor.
Protein Families E.coli NlpD/Haemophilus LppB family
Database Links

KEGG: ecj:JW2712

STRING: 316385.ECDH10B_2910

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