Recombinant Escherichia coli O157:H7 Secreted effector protein EspF(U)

Product Type Recombinant Protein
Uniprot No. C6UYI3
Species Escherichia coli O157:H7 (strain TW14359 / EHEC)
Gene Names espF(U)
Protein Names Recommended name: Secreted effector protein EspF(U) Alternative name(s): EspF-like protein encoded on prophage U Tir-cytoskeleton coupling protein TccP
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion (By similarity).
Subcellular Location Secreted, Host cytoplasm
Protein Families EspF(U)/TccP family
Database Links

KEGG: etw:ECSP_2584

Product Details for Different Expression Systems

Product Name Recombinant Escherichia coli O157:H7 Secreted effector protein EspF(U),Yeast
Code CSB-YP512027EOF
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MINNVSSLFP TVNRNITAVY KKSSFSVSPQ KITLNPVKIS SPFSPSSSSI SATTLFRAPN AHSASFHRQS TAESSLHQQL PNVRQRLIQH LAEHGIKPAR SMAEHIPPAP NWPAPPPPVQ NEQSRPLPDV AQRLVQHLAE HGIQPARNMA EHIPPAPNWP APPLPVQNEQ SRPLPDVAQR LVQHLAEHGI QPARSMAEHI PPAPNWPAPP PPVQNEQSRP LPDVAQRLMQ HLAEHGIQPA RNMAEHIPPA PNWPAPTPPV QNEQSRPLPD VAQRLMQHLA EHGIQPARNM AEHIPPAPNW PAPTPPVQNE QSRPLPDVAQ RLMQHLAEHG INTSKRS
Expression Region 1-337
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Escherichia coli O157:H7 Secreted effector protein EspF(U),E.coli
Code CSB-EP512027EOF
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MINNVSSLFP TVNRNITAVY KKSSFSVSPQ KITLNPVKIS SPFSPSSSSI SATTLFRAPN AHSASFHRQS TAESSLHQQL PNVRQRLIQH LAEHGIKPAR SMAEHIPPAP NWPAPPPPVQ NEQSRPLPDV AQRLVQHLAE HGIQPARNMA EHIPPAPNWP APPLPVQNEQ SRPLPDVAQR LVQHLAEHGI QPARSMAEHI PPAPNWPAPP PPVQNEQSRP LPDVAQRLMQ HLAEHGIQPA RNMAEHIPPA PNWPAPTPPV QNEQSRPLPD VAQRLMQHLA EHGIQPARNM AEHIPPAPNW PAPTPPVQNE QSRPLPDVAQ RLMQHLAEHG INTSKRS
Expression Region 1-337
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Escherichia coli O157:H7 Secreted effector protein EspF(U),Baculovirus
Code CSB-BP512027EOF
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MINNVSSLFP TVNRNITAVY KKSSFSVSPQ KITLNPVKIS SPFSPSSSSI SATTLFRAPN AHSASFHRQS TAESSLHQQL PNVRQRLIQH LAEHGIKPAR SMAEHIPPAP NWPAPPPPVQ NEQSRPLPDV AQRLVQHLAE HGIQPARNMA EHIPPAPNWP APPLPVQNEQ SRPLPDVAQR LVQHLAEHGI QPARSMAEHI PPAPNWPAPP PPVQNEQSRP LPDVAQRLMQ HLAEHGIQPA RNMAEHIPPA PNWPAPTPPV QNEQSRPLPD VAQRLMQHLA EHGIQPARNM AEHIPPAPNW PAPTPPVQNE QSRPLPDVAQ RLMQHLAEHG INTSKRS
Expression Region 1-337
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Escherichia coli O157:H7 Secreted effector protein EspF(U),Mammalian cell
Code CSB-MP512027EOF
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MINNVSSLFP TVNRNITAVY KKSSFSVSPQ KITLNPVKIS SPFSPSSSSI SATTLFRAPN AHSASFHRQS TAESSLHQQL PNVRQRLIQH LAEHGIKPAR SMAEHIPPAP NWPAPPPPVQ NEQSRPLPDV AQRLVQHLAE HGIQPARNMA EHIPPAPNWP APPLPVQNEQ SRPLPDVAQR LVQHLAEHGI QPARSMAEHI PPAPNWPAPP PPVQNEQSRP LPDVAQRLMQ HLAEHGIQPA RNMAEHIPPA PNWPAPTPPV QNEQSRPLPD VAQRLMQHLA EHGIQPARNM AEHIPPAPNW PAPTPPVQNE QSRPLPDVAQ RLMQHLAEHG INTSKRS
Expression Region 1-337
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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