Recombinant Escherichia coli O157:H7 Secreted effector protein EspF(U)

Code CSB-YP512027EOF
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Source Yeast
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Code CSB-EP512027EOF
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Source E.coli
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Code CSB-EP512027EOF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP512027EOF
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Source Baculovirus
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Code CSB-MP512027EOF
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names espF(U)
Uniprot No. C6UYI3
Alternative Names espF(U); tccP; ECSP_2584Secreted effector protein EspF(U; EspF-like protein encoded on prophage U; Tir-cytoskeleton coupling protein TccP
Species Escherichia coli O157:H7 (strain TW14359 / EHEC)
Expression Region 1-337
Target Protein Sequence MINNVSSLFP TVNRNITAVY KKSSFSVSPQ KITLNPVKIS SPFSPSSSSI SATTLFRAPN AHSASFHRQS TAESSLHQQL PNVRQRLIQH LAEHGIKPAR SMAEHIPPAP NWPAPPPPVQ NEQSRPLPDV AQRLVQHLAE HGIQPARNMA EHIPPAPNWP APPLPVQNEQ SRPLPDVAQR LVQHLAEHGI QPARSMAEHI PPAPNWPAPP PPVQNEQSRP LPDVAQRLMQ HLAEHGIQPA RNMAEHIPPA PNWPAPTPPV QNEQSRPLPD VAQRLMQHLA EHGIQPARNM AEHIPPAPNW PAPTPPVQNE QSRPLPDVAQ RLMQHLAEHG INTSKRS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion (By similarity).
Subcellular Location Secreted, Host cytoplasm
Protein Families EspF(U)/TccP family
Database Links

KEGG: etw:ECSP_2584

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