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Recombinant Escherichia coli Proline/betaine transporter (proP), partial

Recombinant Escherichia coli Proline/betaine transporter (proP), partial

Code	CSB-YP314757ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP314757ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP314757ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP314757ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP314757ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

proP

Uniprot No.

P0C0L7

Alternative Names

proP; b4111; JW4072; Proline/betaine transporter; Proline porter II; PPII

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in the bacterial osmoregulatory response.

Gene References into Functions

molecular model predicts the presence of a proton pathway within the N-terminal six-helix bundle of ProP PMID: 15823022
ProP osmotic activation is tuned by both its C-terminal coiled-coil and osmotically induced changes in phospholipid composition PMID: 16239220
These studies define position of ProP TM XII within the membrane, further support predicted structure of ProP, reveal the dimerization interface, and show that the structure of transmembrane segment XII influences the osmolality at which ProP activates. PMID: 17441691
Association of proP with cardiolipin (and not phosphatidylglycerol) may be required for polar ProP localization. PMID: 17504273
ProP switches among "off", "on", and "osmolality-sensitive" states as the membrane potential increases. PMID: 18620422
Periplasmic loops of osmosensory transporter ProP in Escherichia coli are sensitive to osmolality PMID: 19049385
ProP adopts a highly alpha-helical fold similar to that of LacY, and both transmembrane folds exhibit unusually high temperature-sensitive solvent accessibility PMID: 19366597