Recombinant Escherichia coli Protein phnP (phnP)

Code CSB-YP323452ENV
MSDS
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Source Yeast
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Code CSB-EP323452ENV
MSDS
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Source E.coli
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Code CSB-EP323452ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323452ENV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP323452ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
phnP
Uniprot No.
Alternative Names
phnP; b4092; JW4053; Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase; EC 3.1.4.55; Phosphoribosyl cyclic phosphodiesterase
Species
Escherichia coli (strain K12)
Expression Region
1-252
Target Protein Sequence
MSLTLTLTGT GGAQGVPAWG CECAACARAR RSPQYRRQPC SGVVKFNDAI TLIDAGLHDL ADRWSPGSFQ QFLLTHYHMD HVQGLFPLRW GVGDPIPVYG PPDEQGCDDL FKHPGLLDFS HTVEPFVVFD LQGLQVTPLP LNHSKLTFGY LLETAHSRVA WLSDTAGLPE KTLKFLRNNQ PQVMVMDCSH PPRADAPRNH CDLNTVLALN QVIRSPRVIL THISHQFDAW LMENALPSGF EVGFDGMEIG VA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the hydrolysis of the cyclic ribose-phosphate to form alpha-D-ribose 1,5-bisphosphate.
Gene References into Functions
  1. Alteration of H200 to alanine in PhnP resulted in a 6-33-fold decrease in activity. PMID: 21830807
  2. the role of PhnP was examined by (31)P NMR spectrometry on the culture supernatants of E. coli phn mutants grown in the presence of alkylphosphonic acid or phosphite. PMID: 21341651
  3. The overall fold of PhnP is very similar to that of the tRNase Z endonucleases but lacks the long exosite module used by these enzymes to bind their tRNA substrates. PMID: 19366688
Database Links
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