Recombinant Escherichia coli Putative polysaccharide export protein wza (wza)

Code CSB-YP364266ENV
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Source Yeast
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Code CSB-EP364266ENV
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Source E.coli
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Code CSB-EP364266ENV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP364266ENV
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Source Baculovirus
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Code CSB-MP364266ENV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
wza
Uniprot No.
Alternative Names
wza; b2062; JW2047; Putative polysaccharide export protein Wza
Species
Escherichia coli (strain K12)
Expression Region
21-379
Target Protein Sequence
CTVLPGSNMS TMGKDVIKQQ DADFDLDKMV NVYPLTPRLI DQLRPRPNVA RPNMTLESEI ANYQYRVGPG DVLNVTVWDH PELTTPAGQY RSSSDTGNWV QPDGTMFYPY IGKVHVVGKT LAEIRSDITG RLATYIADPQ VDVNIAAFRS QKAYISGQVN KSGQQAITNV PLTILDAINA AGGLTDTADW RNVVLTHNGR EERISLQALM QNGDLNQNRL LYPGDILYVP RNDDLKVFVM GEVKKQSTLK MDFSGMTLTE ALGNAEGIDM TTSNASGIFV IRPLKGEGGR NGKIANIYQL DMSDATSLVM ATEFRLQPYD VVYVTTAPVS RWNRLINQLL PTISGVRYMT DTASDIHNW
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably involved in the export of the extracellular polysaccharide colanic acid from the cell to medium.
Gene References into Functions
  1. 2.26 A resolution structure of the 340 kDa octamer of Wza, an integral outer membrane lipoprotein, which is essential for group 1 capsule export in Escherichia coli PMID: 17086202
  2. the Wza-Wzc complex provides the structural and regulatory core of a larger macromolecular machine, suggesting a mechanism by which capsular polysaccharides may move from the periplasm through the outer membrane PMID: 17283336
Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
BexD/CtrA/VexA family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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