Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

rpoH

Uniprot No.

P0AGB3

Research Area

Others

Alternative Names

rpoH; fam; hin; htpR; b3461; JW3426; RNA polymerase sigma factor RpoH; Heat shock regulatory protein F33.4; RNA polymerase sigma-32 factor

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-284aa

Target Protein Sequence

MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYHGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

39.9 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Escherichia coli (strain K12) rpoH covers amino acids 1-284. The expected molecular weight for the rpoH protein is calculated to be 39.9 kDa. This rpoH protein is produced using e.coli expression system. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of rpoH, which enables a simple process of detecting and purifying the rpoH recombinant protein in the following steps.

The Escherichia coli RNA polymerase sigma factor RpoH is a key regulator involved in the bacterial heat shock response. RpoH is responsible for directing RNA polymerase to transcribe genes that encode heat shock proteins, which are essential for the cell's survival under conditions of elevated temperature or other stressors. In non-stress conditions, RpoH is present at low levels in the cell. However, when exposed to heat or other stressful conditions, the concentration of RpoH increases, leading to its association with RNA polymerase and subsequent activation of the transcription of heat shock genes. These heat shock proteins assist in the proper folding and refolding of proteins, preventing denaturation and aggregation, and helping the cell to adapt and survive stressful conditions. The study of RpoH and the heat shock response provides insights into the cellular mechanisms that bacteria employ to cope with environmental challenges.

Target Background

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. Intracellular concentration of free RpoH protein increases in response to heat shock, which causes association with RNA polymerase (RNAP) and initiation of transcription of heat shock genes, including numerous global transcriptional regulators and genes involved in maintaining membrane functionality and homeostasis. RpoH is then quickly degraded, leading to a decrease in the rate of synthesis of heat shock proteins and shut-off of the heat shock response.

Gene References into Functions

ThiS, an ubiquitin-like protein, is covalently attached to delta(32) and at least 27 other proteins, leading to their subsequent degradation by proteases, in a similar manner to the ubiquitin-proteasome system (UPS) in eukaryotes. PMID: 25721662
co-opts the signal recognition particle to regulate protein homeostasis PMID: 24358019
Authors conclude that oxidative stress triggers the heat shock response in Escherichia coli sigma(32) dependently. PMID: 23585533
N-terminal half of region 2.1 is required for modulating both metabolic stability and the activity of sigma32 PMID: 15516558
First systematic study of the sigma 32 regulon in E. coli including sigma 32 protein level determination gives insight into the complex network regulated by sigma 32 and how it contributes physiological adaptation to changes in the external environment. PMID: 15757896
response protects the cell membrane and responds to its status: Fully 25% of sigma32 regulon members reside in the membrane and alter its functionality; a disproportionate fraction of overexpressed proteins that induce the response are membrane localized PMID: 16818608
Mutants identify a regulatory step downstream of chaperone binding that is required for both inactivation and degradation of sigma32. PMID: 17968012
sigma32-mediated gene expression is a general response to protein insolubility in E. coli PMID: 17992580
it is deduced from these data that there may be a link between sigma(32) regulon activity and cell division events PMID: 19114495
The -10 region in sigma32 is a composite element required for high promoter activity. PMID: 19400791

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Subcellular Location

Cytoplasm.

Protein Families

Sigma-70 factor family, RpoH subfamily

Database Links

KEGG: ecj:JW3426

STRING: 316385.ECDH10B_3635

Code	CSB-EP313777ENV
Abbreviation	Recombinant E.coli rpoH protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Escherichia coli RNA polymerase sigma factor RpoH (rpoH)

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