Code | CSB-EP313777ENV |
Size | US$388 |
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The expression region of this recombinant Escherichia coli (strain K12) rpoH covers amino acids 1-284. The expected molecular weight for the rpoH protein is calculated to be 39.9 kDa. This rpoH protein is produced using e.coli expression system. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of rpoH, which enables a simple process of detecting and purifying the rpoH recombinant protein in the following steps.
The Escherichia coli RNA polymerase sigma factor RpoH is a key regulator involved in the bacterial heat shock response. RpoH is responsible for directing RNA polymerase to transcribe genes that encode heat shock proteins, which are essential for the cell's survival under conditions of elevated temperature or other stressors. In non-stress conditions, RpoH is present at low levels in the cell. However, when exposed to heat or other stressful conditions, the concentration of RpoH increases, leading to its association with RNA polymerase and subsequent activation of the transcription of heat shock genes. These heat shock proteins assist in the proper folding and refolding of proteins, preventing denaturation and aggregation, and helping the cell to adapt and survive stressful conditions. The study of RpoH and the heat shock response provides insights into the cellular mechanisms that bacteria employ to cope with environmental challenges.There are currently no reviews for this product.
KEGG: ecj:JW3426
STRING: 316385.ECDH10B_3635