Name: rpoH Antibody
Brand: CUSABIO
SKU: CSB-PA313777XA01ENV

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rpoH Polyclonal antibody

Uniprot No.

P0AGB3

Target Names

rpoH

Alternative Names

rpoH antibody; fam antibody; hin antibody; htpR antibody; b3461 antibody; JW3426 antibody; RNA polymerase sigma factor RpoH antibody; Heat shock regulatory protein F33.4 antibody; RNA polymerase sigma-32 factor antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) rpoH protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. Intracellular concentration of free RpoH protein increases in response to heat shock, which causes association with RNA polymerase (RNAP) and initiation of transcription of heat shock genes, including numerous global transcriptional regulators and genes involved in maintaining membrane functionality and homeostasis. RpoH is then quickly degraded, leading to a decrease in the rate of synthesis of heat shock proteins and shut-off of the heat shock response.

Gene References into Functions

ThiS, an ubiquitin-like protein, is covalently attached to delta(32) and at least 27 other proteins, leading to their subsequent degradation by proteases, in a similar manner to the ubiquitin-proteasome system (UPS) in eukaryotes. PMID: 25721662
co-opts the signal recognition particle to regulate protein homeostasis PMID: 24358019
Authors conclude that oxidative stress triggers the heat shock response in Escherichia coli sigma(32) dependently. PMID: 23585533
N-terminal half of region 2.1 is required for modulating both metabolic stability and the activity of sigma32 PMID: 15516558
First systematic study of the sigma 32 regulon in E. coli including sigma 32 protein level determination gives insight into the complex network regulated by sigma 32 and how it contributes physiological adaptation to changes in the external environment. PMID: 15757896
response protects the cell membrane and responds to its status: Fully 25% of sigma32 regulon members reside in the membrane and alter its functionality; a disproportionate fraction of overexpressed proteins that induce the response are membrane localized PMID: 16818608
Mutants identify a regulatory step downstream of chaperone binding that is required for both inactivation and degradation of sigma32. PMID: 17968012
sigma32-mediated gene expression is a general response to protein insolubility in E. coli PMID: 17992580
it is deduced from these data that there may be a link between sigma(32) regulon activity and cell division events PMID: 19114495
The -10 region in sigma32 is a composite element required for high promoter activity. PMID: 19400791

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Sigma-70 factor family, RpoH subfamily

Database Links

KEGG: ecj:JW3426

STRING: 316385.ECDH10B_3635

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rpoH Antibody

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