Recombinant Escherichia coli RNA polymerase sigma factor rpoD (rpoD)

1. Counter-intuitively, intermolecular epistasis can alleviate the constraints of individual components, thereby increasing phenotypic variation that selection could act on and facilitating adaptive evolution. PMID: 29130883
2. In contrast to NusG, which freely binds to RNA polymerase, RfaH exists in a structurally distinct autoinhibitory state in which the RNA polymerase-binding site is buried at the interface between two RfaH domains. PMID: 28605514
3. Here the authors establish that the primary sigma factor in Escherichia coli, sigma(70), can function as an elongation factor in vivo by loading directly onto the transcription elongation complex in trans. PMID: 26371553
4. These data suggested that template strand single-stranded DNA competes with lipiarmycin for binding to RNA polymerase and that sigma(70) regions 1.2 and 3.2 attenuate lipiarmycin action by promoting DNA duplex opening. PMID: 26724534
5. The sigma70-subunit of E. coli RNA polymerase (a small protein, being a part of RNA holoenzyme, and responsible for initiation of transcription of constitutive genes) is modeled at different ionic strengths PMID: 26841497
6. Data suggest that RNAP sigma 70 interactions with DNA immediately downstream of the transcription bubble are particularly important for initiation of transcription. PMID: 25311862
7. Authors conclude that bacteriophage interruption of mlrA and RpoS mutations provide major obstacles limiting curli expression and biofilm formation in most serotype O157 : H7 strains. PMID: 23744902
8. where at least three regions of the 6S RNA must interact with Esigma(70) in a cooperative manner so as to ensure effective pRNA-dependent release. PMID: 24681966
9. The study presents the initial steps of the 6S RNA - RNA polymerase complex decay. PMID: 23667906
10. X-ray crystal structure of Escherichia coli RNA polymerase sigma70 holoenzyme PMID: 23389035
11. Deliberately slowing the intrinsic 6S RNA release rate by nucleotide feeding experiments reveals that sigma70 ejection occurs abruptly once a pRNA length of 9 nucleotides is reached. PMID: 23118417
12. study found that although Rob makes fewer interactions with sigma(70) R4 than SoxS, the 2 proteins make the same position-dependent interactions; Rob occludes sigma(70) R4 from binding the -35 hexamer, just as does SoxS; thus, the C-terminal domain does not substantially alter the way Rob interacts with sigma(70) R4 at class II promoters PMID: 22465792
13. Data indicate that region 1.1 and, to a lesser extent, region 1.2 of sigma(70) subunit determine higher promoter complex stability of RNA polymerase (RNAP). PMID: 22605342
14. changes in the structure of region 2 of the sigma(70) subunit have stronger effects on transcription pausing than on promoter recognition, likely by weakening the interactions of the sigma subunit with the core RNAP during transcription elongation. PMID: 22098235
15. The strength of the -15 element in sigma(70) promoter inversely correlates with the strengths of the -35 element and -10 element. PMID: 21908667
16. sigma70 drives yjbEFGH transcription and it also acts to repress its induction. PMID: 21396442
17. Mutation of sigma(70) residue R451, which is highly conserved, results in reduced growth rate, consistent with a central role in promoter recognition. PMID: 21398630
18. His180 substitutions influence several steps of transcription initiation, including core binding, promoter DNA recognition and open complex formation. PMID: 21451247
19. Genetic evidence for a novel interaction between transcriptional activator soxs and region 4 of the sigma(70) subunit of RNA polymerase at class ii SoxS-dependent promoters in Escherichia coli PMID: 21195716
20. SoxS is now the first example of an E. coli transcriptional activator that uses a single positive control surface to make specific protein-protein contacts with two different subunits of RNAP. PMID: 20595001
21. Phenotypically, cells overproducing sigma(70) favours growth and reproduction at the expense of motility and damage protection. PMID: 19574956
22. in vivo association of sigma(70) and NusA with elongating RNA polymerase is regulated by growth conditions PMID: 15596728
23. analysis of promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerases PMID: 15731103
24. amino acid substitutions in region 4 of sigma(70) that have specific effects on the sigma(70) region 4/beta-flap RNA polymerase interaction, either weakening or strengthening it PMID: 15761057
25. Establishment of downstream contacts leads to remodeling of upstream interactions between sigma70 and the -10 promoter element that might facilitate promoter escape and sigma release PMID: 15978618
26. the recognition of the -35 region involves similar amino acid residues in regions 4.2 of E. coli sigma32 and sigma70 PMID: 16166539
27. the core of promoter melting activity of RNA polymerase is localized to a very small subset of all promoter-polymerase contacts PMID: 16169843
28. an important role of sigma region 3.2 in the binding of initiating substrates in RNA polymerase active center and in the process of promoter clearance PMID: 16690607
29. Sigma region 1.2 acts as a core RNA polymerase-dependent allosteric switch that modulates non-template DNA strand recognition by sigma region 2 during transcription initiation and elongation. PMID: 17268549
30. sigma(70) NCR/beta' interaction facilitates promoter escape and hinders early elongation pausing, in contrast to the sigma(2)/beta' coiled-coil interaction, which has opposite effects. PMID: 17332752
31. experiments support a model in which the role of Rsd is primarily to sequester sigma(70), thereby increasing the levels of RNA polymerase containing the alternative sigma(38) factor PMID: 17351046
32. The 2.6 A-resolution X-ray crystal structure of Rsd bound to sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding within sigma(70), was determined. PMID: 17681541
33. the T429 of Sigma 70 acts as a competitor for the hydrogen bonding that stabilizes the highly conserved - 11A-T base pairs of the promoter DNA, thus facilitating initiation of strand separation at this particular position in the - 10 region. PMID: 18155246
34. The tyrosine at residue 430 in region 2.3 of sigma(70) was shown to be involved in quenching the fluorescence of a 2-AP substituted at -11, presumably through a stacking interaction. PMID: 18976666
35. Residues in sigma70 region 4.2, the C-terminus of the second helix, contribute significantly to 6S RNA binding but not to binding of a minimal promoter. PMID: 19538447

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KEGG: ecj:JW3039

STRING: 316385.ECDH10B_3242