Recombinant Escherichia coli Ribonuclease HI (rnhA)

Code CSB-YP359051ENV
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP359051ENV
MSDS
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Source E.coli
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Code CSB-EP359051ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP359051ENV
MSDS
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Source Baculovirus
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Code CSB-MP359051ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rnhA
Uniprot No.
Alternative Names
rnhA; dasF; herA; rnh; sdrA; b0214; JW0204; Ribonuclease HI; RNase HI; EC 3.1.26.4; Ribonuclease H; RNase H
Species
Escherichia coli (strain K12)
Expression Region
1-155
Target Protein Sequence
MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK EHCEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW VKGHAGHPEN ERCDELARAA AMNPTLEDTG YQVEV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.
Gene References into Functions
  1. Data show that the catalytic domains of E. coli and human RNase H have nearly identical sequence preferences, which correlate with the efficiency of RNase H-recruiting antisense oligonucleotides. PMID: 29126318
  2. RNase HI recruits RnlB to RnlA through N-repeated domain for inhibiting RnlA toxicity and thus plays two contrary roles in the regulation of RnlA-RnlB system. PMID: 28085056
  3. Here, authors provide the evidence that RNase HI is an essential factor for activation of RnlA. PMID: 24308852
  4. overproduction compensates for absence of topoisomerase I in RNA synthesis PMID: 15458416
  5. crystal structure of ribonuclease HI PMID: 15644213
  6. report of the complete force-induced unfolding and refolding trajectory of a single molecule of RNase H with the use of force-measuring optical tweezers PMID: 16179479
  7. Results describe the unfolded state ensemble of RNAse H. PMID: 16426636
  8. Adjusting conformational changes within RNase H for activity at high temperatures. PMID: 17088323
  9. RNase HI plays a more central role in DNA topology than previously thought. PMID: 18554330
  10. Data show that overproduction of RNase HI limits the accumulation of hypernegative supercoiling, thereby preventing extensive RNA degradation. PMID: 18790862
  11. a single change at Ile25 (I25A) resulted in the equilibrium population of the RNase H intermediate under near-native conditions. PMID: 19505477

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Subcellular Location
Cytoplasm.
Protein Families
RNase H family
Database Links
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