Recombinant Escherichia coli Sec-independent protein translocase protein TatA (tatA), partial

Code CSB-YP300837ENV
MSDS
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Source Yeast
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Code CSB-EP300837ENV
MSDS
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Source E.coli
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Code CSB-EP300837ENV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP300837ENV
MSDS
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Source Baculovirus
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Code CSB-MP300837ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tatA
Uniprot No.
Alternative Names
tatA; mttA1; yigT; b3836; JW3813; Sec-independent protein translocase protein TatA
Species
Escherichia coli (strain K12)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.
Gene References into Functions
  1. The authors observe that substitutions of charged residues located in the TatA amphipathic helix lock TatA in an assembled state, suggesting that these charged residues play a critical role in the protein translocation step that follows TatA assembly. PMID: 28857741
  2. Live cell imaging shows reversible assembly of the TatA component of the twin-arginine protein transport system. PMID: 24003141
  3. Each subunit of TatA consists of a transmembrane segment, an amphiphilic helix (APH), and a C-terminal densely charged region (DCR). The sequence of charges in the DCR is complementary to the charge pattern on the APH, suggesting that the protein can be "zipped up" by a ladder of seven salt bridges. PMID: 23332763
  4. analysis of the Tat translocation mechanism through structural and biophysical studies of the amphipathic region of TatA from Escherichia coli PMID: 21683683
  5. the TatA protein represents a bifunctional component fulfilling both the TatA and TatB functions. PMID: 15557327
  6. TatA fused to green/cyan/yellow fluorescent protein is found to be preferentially incorporated into the TatABC complex. PMID: 15728576
  7. TatA may be the major pore-forming subunit in the twin-arginine translocation pathway [review] PMID: 15748976
  8. We used purified preproteins to study interaction with the phospholipid membrane by using surface plasmon resonance.Preproteins interact tightly with a model membrane consisting of only phospholipids in early step in protein translocation by Tat system. PMID: 16475812
  9. it is only in TatA that the amphipathic helix is sensitive to amino acid substitutions; the TatA amphipathic helix may play a role in forming and controlling the path of substrate movement across the membrane PMID: 17565984
  10. data support the TatA polymerization model for the mechanism of Tat transport. PMID: 18832162

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Subcellular Location
Cell inner membrane; Single-pass membrane protein; Cytoplasmic side. Note=Abundant all over the membrane, but is more concentrated at the cell poles.
Protein Families
TatA/E family
Database Links
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