Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
talB; yaaK; b0008; JW0007; Transaldolase B; EC 2.2.1.2
Species
Escherichia coli (strain K12)
Expression Region
2-317aa
Target Protein Sequence
TDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQIPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLSYDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFSFAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGYETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARITESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 2-317 form the expressed segment for recombinant Escherichia coli (strain K12) talB. The theoretical molecular weight of the talB protein is 42.5 kDa. This talB recombinant protein is manufactured in e.coli. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of talB, which enables a simple process of detecting and purifying the talB recombinant protein in the following steps.
Transaldolase B (TalB) in Escherichia coli is an enzyme involved in the non-oxidative phase of the pentose phosphate pathway (PPP), a metabolic pathway crucial for the production of ribose-5-phosphate and NADPH. TalB specifically catalyzes the reversible transfer of a three-carbon dihydroxyacetone moiety from the ketose donor fructose-6-phosphate to the aldose acceptor erythrose-4-phosphate. This reaction results in the formation of sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate. The pentose phosphate pathway plays a vital role in balancing the cell's need for ribose-5-phosphate, a precursor for nucleotide biosynthesis, and generating reducing equivalents in the form of NADPH. TalB's involvement in this pathway positions it at the intersection of central carbon metabolism and nucleotide biosynthesis, making it crucial for cellular growth and homeostasis. Understanding the functions of TalB contributes to the broader knowledge of bacterial metabolism and has potential applications in metabolic engineering for the production of biofuels or other valuable compounds.
