Recombinant Escherichia coli Vitamin B12 transporter BtuB (btuB), partial

Code CSB-YP361723ENV
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Source Yeast
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Code CSB-EP361723ENV
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Source E.coli
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Code CSB-EP361723ENV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP361723ENV
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Source Baculovirus
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Code CSB-MP361723ENV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
btuB
Uniprot No.
Alternative Names
btuB; bfe; cer; dcrC; b3966; JW3938Vitamin B12 transporter BtuB; Cobalamin receptor; Outer membrane cobalamin translocator
Species
Escherichia coli (strain K12)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB. Is also a receptor for bacteriophages BF23 and C1, and for A and E colicins.
Gene References into Functions
  1. The btuB riboswitch modulates the formation of a tertiary structure to perform metabolite sensing and regulate gene expression. PMID: 26370077
  2. The lack of a AdoCbl-induced conformational switch experienced by the btuB riboswitch in the absence of Mg(2+) indicates a crucial role played by Mg(2+) for defining an active conformation of the riboswitch. PMID: 24243114
  3. A portion of the N-terminal region of BtuB which occupies the lumen of the BtuB barrel denatures prior to the unfolding of the BtuB barrel, so that the free energy of folding for the N-terminus is smaller than that typically seen for globular proteins. PMID: 22497281
  4. analysis of the conformation of BtuB by electron paramagnetic resonance PMID: 21894979
  5. Data describe the effect of calcium ions on the conformation of the extracellular loops of BtuB and their possible role in B12 binding. PMID: 19927326
  6. The functional relevance of the five C-terminal loops of the cobalamin transporter BtuB was examined. PMID: 15716445
  7. the N-terminal end of the Ton box(a highly conserved segment near the N-terminus of the btuB) extends approximately 20 to 30 A into the periplasm upon the addition of substrate B12 PMID: 16953570
  8. Crystals of BtuB that diffract to 1.95 A have been grown by the membrane-based in meso technique; protein structure differs in several details from that of its counterpart grown by the more traditional, detergent-based method. PMID: 17028020
  9. The data indicate that charged residues (D316 and R14) are critical to the unfolding and conformational equilibrium of the energy-coupling motif. PMID: 17555764

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Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
TonB-dependent receptor family, BtuB (TC 1.B.14.3.1) subfamily
Database Links
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