Code | CSB-EP020656CA |
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Size | US$388 |
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The production of the recombinant Felis catus SAA1 protein begins with the creation of the recombinant plasmid, which is synthesized by inserting the gene encoding the Felis catus SAA1 protein (1-90aa) into a plasmid vector. The recombinant plasmid is introduced into e.coli cells. e.coli cells that can survive in the presence of a specific antibiotic are selected and then cultured under conditions conducive to the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-B2M tag. Following expression, the recombinant SAA1 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is then employed to resolve the resulting recombinant Felis catus SAA1 protein, demonstrating a purity exceeding 90%.
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The sequence corresponds to beginning of the mature chain - 96% homology with aa19-111 rather than aa1-90 as stated on datasheet. Please can you confirm the sequence is correct.
STRING: 9685.ENSFCAP00000020110