Recombinant Flock house virus Protein B2 (B2)

Code CSB-YP304757FCA
MSDS
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Source Yeast
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Code CSB-EP304757FCA
MSDS
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Source E.coli
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Code CSB-EP304757FCA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP304757FCA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP304757FCA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
B2
Uniprot No.
Alternative Names
B2Protein B2
Species
Flock house virus (FHV)
Expression Region
1-106
Target Protein Sequence
MPSKLALIQE LPDRIQTAVE AAMGMSYQDA PNNVRRDLDN LHACLNKAKL TVSRMVTSLL EKPSVVAYLE GKAPEEAKPT LEERLRKLEL SHSLPTTGSD PPPAKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.
Gene References into Functions
  1. B2 binding to double-stranded regions of RNA2 prevents recruitment of RNA2 into cellular structures. PMID: 24089564
  2. Our data provide evidence that both monomeric and dimeric B2 proteins function in different modes to suppress RNA silencing. PMID: 22721960
  3. These results reveal a novel interaction between Flock house virus B2 protein and Dicer that leads to suppression of siRNA biogenesis. PMID: 19193719
Protein Families
Nodaviridae B2 protein family
Database Links

KEGG: vg:1724847

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