Name: Recombinant Halobacterium salinarum Cobalamin-binding protein (btuF)
Brand: CUSABIO
SKU: 534746HTL

Product Details

Purity

>85% (SDS-PAGE)

Target Names

btuF

Uniprot No.

B0R5G2

Alternative Names

btuF; OE_2951R; Cobalamin-binding protein; Vitamin B12-binding protein

Species

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)

Expression Region

25-369

Target Protein Sequence

LAPQPP AQHADADRAC SFPVTEPDAS NTAITLDSEP ERVVTLNPSA AQTMWELGDR DAVVGVSQFG TYLPTASQRT VVSGGQPSQT NVEAVVGLDP DLVLAPNTVR NTTVTRLRSA GITVFQFRAA TSIDGVVEKT ATIGRLTGNC AAAAATTAEM RDRVAAIADA VPDTDSARPR VYYHLGDGYT AGPNTFIGAA IEAAGGHNIA ADVNTTSSYP QLSEEVIVSQ DPDVVVTGVS ADRLDATASA LVAPSSVVRN TTAYATGNVV AVNTNHINQP APRIVEPMAR MANAFHNTTI NTTLDAQPSA TTTATSTAPP TDAADGTAPG FGVAAAVCAL AGAALVARR

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I had a couple of questions regarding your product CSB-YP534746HTL:
1.I plan to use this protein in AFM imaging experiments. Do you think aseptic processing and/or endotoxin removal are necessary for these experiments?
2.Can you advise whether or not this protein is suitable for lyophilization?
3.CSB-YP534746HTL (yeast), possible to have the tag removed?

Very nice to receive your inquiry.
1.We think aseptic processing or endotoxin removal are not necessary for these experiments.
2.Glycerol will be added into the buffer for liquid protein. If the glycerol will affect your experiments, lyophilized protein is suitable.
3.CSB-YP534746HTL (yeast), possible to have the tag removed?
If you need to remove the tag of this protein, lead time will be extended for 3 business days. And we will charge for tag removal service, here it is the details about the Tag Removal Service:
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”

Target Background

Function

Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably binds cobalamin and delivers it to the surface of BtuC.

Database Links

KEGG: hsl:OE_2951R

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Recombinant Halobacterium salinarum Cobalamin-binding protein (btuF)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

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