Recombinant Helicobacter pylori Cytotoxicity-associated immunodominant antigen (cagA), partial

The recombinant Helicobacter pylori cagA was expressed with the amino acid range of 918-1147. This cagA protein is expected to have a theoretical molecular weight of 41.3 kDa. This cagA recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of cagA, which enables a simple process of detecting and purifying the cagA recombinant protein in the following steps.

The Helicobacter pylori cytotoxicity-associated immunodominant antigen (cagA) is a crucial virulence factor associated with certain strains of H. pylori, a bacterium that infects the human stomach. CagA is delivered into gastric epithelial cells by a type IV secretion system (T4SS), which is encoded by the cag pathogenicity island (cagPAI). Once inside the host cell, CagA undergoes phosphorylation by host cell kinases, leading to alterations in various cellular signaling pathways. Phosphorylated CagA has been linked to disruption of cell polarity, cell-cell adhesion, and the induction of pro-inflammatory responses. Additionally, chronic infection with cagA-positive H. pylori strains is associated with an increased risk of developing peptic ulcers and gastric cancer. The molecular mechanisms by which CagA influences host cell processes are complex and involve its interactions with various host cell proteins. Understanding the role of CagA in H. pylori pathogenesis is crucial for developing strategies to manage and prevent associated diseases.