Recombinant Homeobox protein engrailed-like ceh-16 (ceh-16)

Code CSB-YP333882CXY
MSDS
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Source Yeast
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Code CSB-EP333882CXY
MSDS
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Source E.coli
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Code CSB-EP333882CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333882CXY
MSDS
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Source Baculovirus
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Code CSB-MP333882CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ceh-16
Uniprot No.
Alternative Names
ceh-16; C13G5.1; Homeobox protein engrailed-like ceh-16
Species
Caenorhabditis elegans
Expression Region
1-187
Target Protein Sequence
MILKFGIERI LSSPYPCPSP TISTPATSPS SISPTFASPN GTPNIASSMY PAWVFSTRYS DRPSAGPRHR KSRKRESTGS SGSSEEEKRP RTAFTGDQLD RLKTEFRESR YLTEKRRQEL AHELGLNESQ IKIWFQNKRA KLKKSTSSVP RDRCSSVTPN PHNHPSIHGG YQLMAQLAKV QARAYMP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional regulator which binds to DNA to regulate gene expression and promote seam cell development and differentiation during embryogenesis. Plays a role in maintaining the boundaries between the lateral rows of seam cells and the ventral and dorsal row of epidermal cells during embryonic development. Negatively regulates the expression of the fusion effector protein eff-1 to prevent seam cell fusion with the dorsal and ventral epidermal cells during embryonic elongation. Positively regulates seam cell self-renewal and expansion during the L2 larval stage to promote seam cell development. This role does not seem to be via regulation of eff-1 expression. Specifically, it is required for the asymmetric division of the V5.p seam cell during the L2 larval stage, and in turn the asymmetric nuclear distribution of pop-1 in V5.p daughter cells.
Gene References into Functions
  1. patterns the embryonic epidermis of Caenorhabditis elegans PMID: 15659483
  2. ceh-16 interacts with the Wnt signaling pathway to control the choice between self-renewal expansion and maintenance division PMID: 19607822
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Engrailed homeobox family
Tissue Specificity
Expressed in seam cells.
Database Links

KEGG: cel:CELE_C13G5.1

STRING: 6239.C13G5.1.2

UniGene: Cel.25260

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