Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PLCZ1

Uniprot No.

Q86YW0

Alternative Names

1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1; 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase zeta-1; MGC149685; NYD SP27; Phosphoinositide phospholipase C zeta 1; Phosphoinositide phospholipase C-zeta-1; Phospholipase C zeta 1; Phospholipase C-zeta-1; PI phospholipase C zeta 1; PLC zeta 1; PLC-zeta-1; PLCZ1; PLCZ1_HUMAN; PLCzeta; Testis development protein NYD SP27; Testis development related NYD SP27; Testis-development protein NYD-SP27

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-415aa

Target Protein Sequence

MEMRWFLSKIQDDFRGGKINLEKTQRLLEKLDIRCSYIHVKQIFKTSDYPVVLSLENHCSTAQQEVMADNLQATFGESLLSDMLDDFPDTLPSPEALKFKILVKNKKIGTLKETHERKGSDKRGDNQDKETGVKKLPGVMLFKKKKTRKLKIALALSDLVIYTKAEKFKSFQHSRLYQQFNENNSIGETQARKLSKLRVHEFIFHTRKFITRIYPKATRADSSNFNPQEFWNIGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYILKPHFLRESKSYFNPSNIKEGMPITLTIRLISGIQLPLTHSSSNKGDSLVIIEVFGVPNDQMKQQTRVIKKNAFSPRWNETFTFIIHVPELALIRFVVEGQGLIAGNEFLGQYTLPLLCMNKGYRRIPLFSRMGESLEPASLFVYVWYVR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

64.5kDa

Protein Length

Full length of isoform 2

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human PLCZ1 contains amino acids 1-415. This PLCZ1 protein is expected to have a theoretical molecular weight of 64.5 kDa. This PLCZ1 protein is produced using e.coli expression system. Fusion of the N-terminal 6xHis-SUMO tag into the PLCZ1 encoding gene fragment was conducted, allowing for easier detection and purification of the PLCZ1 protein in subsequent stages.

Human 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Zeta-1 (PLCZ1) is a key enzyme involved in sperm function. Its primary function is to hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol trisphosphate (IP3), initiating calcium release during sperm-egg fusion. In reproductive research, PLCZ1 is pivotal for understanding fertilization mechanisms, and its dysfunction may contribute to male infertility. Additionally, PLCZ1's role in intracellular signaling implicates it in broader cell biology and signal transduction studies. Investigating PLCZ1 provides insights into reproductive processes, male infertility, and signaling cascades, offering potential applications in assisted reproductive technologies and advancing the understanding of cellular mechanisms related to fertility and intracellular communication.

Target Background

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.

Gene References into Functions

PLCzeta and PAWP impairments may be one of the possible etiologies of decreased fertility in Oligoasthenoteratozoospermia PMID: 28954204
Low PLCZ1 expression is associated with globozoospermia with DPY19L2 deletion. PMID: 29339016
we evaluate sperm motility and the proportion of sperm expressing PLC zeta in 71 males (22-54 years; 44 fertile controls and 27 infertile patients), along with total levels and localisation patterns of PLC zeta within the sperm head. PMID: 27270687
findings suggest that human PLCZ1 RNA is a better therapeutic agent to rescue human oocytes from failed activation, leading to normal and efficient development. PMID: 28320563
The value of PAWP and PLCzeta as indicators of sperm quality was studied. PMID: 28076980
The role of the PLCZ1 protein in male infertility, and its expression regulation and therapeutic potential have been discussed. (Review) PMID: 26700242
latest developments that have begun to unravel the vital role of PLCzeta at mammalian fertilisation and decipher its unique mechanism of action within the fertilising egg. PMID: 29061915
The findings highlight the importance of PLCzeta at fertilization and the vital role of the C2 domain in PLCzeta function, possibly due to its novel binding characteristics. PMID: 28270562
The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCzeta PMID: 27612872
In male infertility, the absence of PLCZ1 alone is sufficient to prevent oocyte activation irrespective of the presence of PAWP. PMID: 26721930
Human PLCzeta elicited the characteristic Ca(2+) oscillations present at mammalian fertilization, which produced oocyte activation and embryo development. PMID: 26116451
Study further supports the fundamental role of PLCzeta in the oocyte activation process. PMID: 26054556
Phospholipase C-zeta deficiency is associated with repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization. PMID: 26174123
I discuss the recent advances in our knowledge of the intriguing biochemical and physiological properties of sperm PLCz and postulate potential roles for PLCz in terms of clinical diagnosis and therapy for certain forms of male infertility PMID: 26009178
Data suggest that PAWP (WW domain-binding protein 2-like) from sperm (rather than PLCZ [phospholipase C zeta] from sperm) acts as the 'sperm factor' within the egg at fertilization and is involved in 'egg activation' and embryogenesis. [REVIEW] PMID: 25722320
in patients with normal SA parameters but with repeated low fertilization or outright failed fertilization results after ICSI, abnormal PLCZ1 function should be considered as the underlying mechanism responsible for the failure of fertilization PMID: 24756570
Human PLCzeta exhibits superior fertilization potency over mouse PLCzeta in triggering the Ca(2+) oscillations required for mammalian oocyte activation. PMID: 24478462
Male infertility is associated with the aberrant expression, localisation, structure and function of PLCzeta in human sperm. [review] PMID: 23916605
PLCzeta may be a significant factor in human fertility with potential therapeutic capacity. [Review] PMID: 24157362
investigation of expression level and localization pattern of PLCZ1 in sperm from three infertile patients with globozoospermia (i.e., patients who exhibited abnormal sperm with round heads) [CASE REPORT] PMID: 22940771
Loss-of-activity mutations in PLCzeta may contribute not only toward male infertility but also male subfertility in cases where PLCzeta is mutated on a single allele. PMID: 22633260
PLCzeta is compartmentalized as part of the acrosome early in human and mouse spermiogenesis and is secreted during sperm maturation in the epididymis. PMID: 22428063
Phospholipase C-zeta-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection. PMID: 22217962
a maternally inherited autosomal point mutation in human phospholipase C zeta (PLCzeta) leads to male infertility PMID: 22095789
PLCzeta may have a role in sperm inducing oocyte activation after ICSI PMID: 21896550
Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLC gamma. PMID: 21204786
confirm the previously proposed inhibitory role of NYD-SP27 in the PLC pathway and demonstrate that the suppression of its expression could result in an enhancement of ATP-stimulated Ca(2+) dependent pancreatic anion secretion. PMID: 17196844
narrow spectrum of PLCZ1 activity indicates that it is modulated by tissue-restricted accessory factors PMID: 17933795
Human PLCzeta can readily activate mouse oocytes, however, effective development to blastocyst stages is only achieved within a specific window of hPLCzeta-luc protein expression levels. PMID: 18003622
All PLCZ1 proteins including fish could induce Ca(2+) oscillations in mouse eggs, but the activity was variable in the order of human >> mouse > medaka >> rat, estimated from minimal RNA concentration to induce Ca(2+) spikes. PMID: 18322275
Human sperm devoid of PLCZ1 fail to induce Ca(2+) release and are unable to initiate the first step of embryo development. PMID: 18924610

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Involvement in disease

Spermatogenic failure 17 (SPGF17)

Subcellular Location

Nucleus. Cytoplasm, perinuclear region.

Tissue Specificity

Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.

Database Links

HGNC: 19218

OMIM: 608075

KEGG: hsa:89869

STRING: 9606.ENSP00000266505

UniGene: Hs.743832

Code	CSB-EP768240HU
Abbreviation	Recombinant Human PLCZ1 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1)

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