Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1; 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase zeta-1; MGC149685; NYD SP27; Phosphoinositide phospholipase C zeta 1; Phosphoinositide phospholipase C-zeta-1; Phospholipase C zeta 1; Phospholipase C-zeta-1; PI phospholipase C zeta 1; PLC zeta 1; PLC-zeta-1; PLCZ1; PLCZ1_HUMAN; PLCzeta; Testis development protein NYD SP27; Testis development related NYD SP27; Testis-development protein NYD-SP27
Species
Homo sapiens (Human)
Expression Region
1-415aa
Target Protein Sequence
MEMRWFLSKIQDDFRGGKINLEKTQRLLEKLDIRCSYIHVKQIFKTSDYPVVLSLENHCSTAQQEVMADNLQATFGESLLSDMLDDFPDTLPSPEALKFKILVKNKKIGTLKETHERKGSDKRGDNQDKETGVKKLPGVMLFKKKKTRKLKIALALSDLVIYTKAEKFKSFQHSRLYQQFNENNSIGETQARKLSKLRVHEFIFHTRKFITRIYPKATRADSSNFNPQEFWNIGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYILKPHFLRESKSYFNPSNIKEGMPITLTIRLISGIQLPLTHSSSNKGDSLVIIEVFGVPNDQMKQQTRVIKKNAFSPRWNETFTFIIHVPELALIRFVVEGQGLIAGNEFLGQYTLPLLCMNKGYRRIPLFSRMGESLEPASLFVYVWYVR
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full length of isoform 2
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Human PLCZ1 contains amino acids 1-415. This PLCZ1 protein is expected to have a theoretical molecular weight of 64.5 kDa. This PLCZ1 protein is produced using e.coli expression system. Fusion of the N-terminal 6xHis-SUMO tag into the PLCZ1 encoding gene fragment was conducted, allowing for easier detection and purification of the PLCZ1 protein in subsequent stages.
Human 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Zeta-1 (PLCZ1) is a key enzyme involved in sperm function. Its primary function is to hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol trisphosphate (IP3), initiating calcium release during sperm-egg fusion. In reproductive research, PLCZ1 is pivotal for understanding fertilization mechanisms, and its dysfunction may contribute to male infertility. Additionally, PLCZ1's role in intracellular signaling implicates it in broader cell biology and signal transduction studies. Investigating PLCZ1 provides insights into reproductive processes, male infertility, and signaling cascades, offering potential applications in assisted reproductive technologies and advancing the understanding of cellular mechanisms related to fertility and intracellular communication.
