Recombinant Human 15 kDa selenoprotein (SEP15)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

SELENOF

Uniprot No.

O60613

Alternative Names

15 kDa selenoprotein; SELENOF; Selenoprotein F; Sep15; SEP15_HUMAN

Species

Homo sapiens (Human)

Expression Region

29-162

Target Protein Sequence

FG AEFSSEACRE LGFSSNLLCS SCDLLGQFNL LQLDPDCRGC CQEEAQFETK KLYAGAILEV CGUKLGRFPQ VQAFVRSDKP KLFRGLQIKY VRGSDPVLKL LDDNGNIAEE LSILKWNTDS VEEFLSEKLE RI

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

May be involved in redox reactions associated with the formation of disulfide bonds. May contribute to the quality control of protein folding in the endoplasmic reticulum. May regulate protein folding by enhancing the catalytic activity of UGGT1/UGCGL1 and UGGT2/UGCGL2.

Gene References into Functions

The allelic and genotypic frequencies did not differ between rapid and nonrapid progressors; however, the presence of the AA genotype of the rs5859 polymorphism was associated with a shorter time of progression to AIDS compared with GG homozygotes (hazard ratio = 3.62, 95% CI = 1.55-8.43, p = 0.003). PMID: 27228552
Genotype variation within the 3'-UTR of the SEP 15 gene showed no association with breast cancer risk or clinico-pathological parameters in Caucasian women. PMID: 26264612
Results suggest that SEP15 plays important roles in the regulation of the G1 phase during the cell cycle as well as in cell motility of Chang liver cells offering a novel functional link between the cell cycle and cell motility. PMID: 25728752
Concerning rs561104, the serum selenium concentration was higher in CC homozygotes (5.65 +/- 1.11 mug/dL) compared with T carriers (4.88 +/- 1.25 mug/dL, P = .044). PMID: 25249019
Sep15 in Chang liver cells regulates the pathway that antagonizes RhoA/ROCK/MLC-dependent non-apoptotic bleb formation. PMID: 25529450
This study provides evidence that SEP15 genetic variation may influence prostate cancer mortality PMID: 20424130
Genetic and functional analysis of mammalian Sep15 selenoprotein. PMID: 11898406
SEP15, encoding a 15-kDa selenium-containing protein was shown to be downregulated in approximately 60% of MM cell lines and tumor specimens. A SEP15 polymorphic variant, 1125A, resides in the SECIS recognition element PMID: 15107826
It appears that those with the Sep15 1125 AA genotype may benefit most from a higher Se intake, whereas in those with the GG or GA genotype, a higher Se status may increase the risk for lung cancer. PMID: 18239845
Studies in mouse show that Sep15 may have redox function. PMID: 12600282

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Subcellular Location

Endoplasmic reticulum lumen. Note=The association with UGGT1/UGCGL1 is essential for its retention in the endoplasmic reticulum.

Protein Families

Selenoprotein M/F family

Tissue Specificity

Higher levels in prostate and thyroid gland.

Database Links

HGNC: 17705

OMIM: 606254

KEGG: hsa:9403

STRING: 9606.ENSP00000328729

UniGene: Hs.362728

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Recombinant Human 15 kDa selenoprotein (SEP15)

Product Details

Customer Reviews and Q&A

Target Background

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