Recombinant Human 26S proteasome non-ATPase regulatory subunit 11 (PSMD11) is produced in E. coli and includes an N-terminal GST tag that makes purification easier. The protein covers the complete mature sequence from amino acids 2 to 422. SDS-PAGE analysis confirms purity levels above 90%, making this product suitable for research applications where consistent results matter.
PSMD11 appears to be a key component of the 26S proteasome—the cellular machinery that breaks down ubiquitinated proteins. This regulatory subunit likely plays an important role in keeping protein levels balanced and controlling different cellular activities. Within the ubiquitin-proteasome pathway, PSMD11 is particularly relevant for studies examining protein breakdown, cell cycle control, and signaling networks.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Proteasome Complex Assembly and Interaction Studies
This recombinant PSMD11 protein may be useful for examining how the 26S proteasome assembles and what proteins interact within this complex. The GST tag allows for pull-down experiments that can reveal binding partners and help map connections with other proteasome components. Scientists can conduct in vitro reconstruction experiments to understand how PSMD11 fits into the 19S regulatory particle and what it contributes to the overall proteasome structure.
2. Antibody Development and Validation
The high-purity recombinant PSMD11 protein works well as an antigen for creating antibodies specific to this proteasome subunit. Since it contains the full-length mature protein (2-422aa), researchers get broad epitope coverage when developing both monoclonal and polyclonal antibodies. The same recombinant protein can then validate these antibodies in Western blots, ELISA assays, and immunoprecipitation experiments.
3. Biochemical Characterization and Structural Studies
This purified PSMD11 protein enables detailed biochemical analysis—things like protein stability, folding characteristics, and identifying sites where post-translational modifications occur. Researchers might use the recombinant protein for structural biology techniques like X-ray crystallography or NMR spectroscopy to determine the three-dimensional shape of this regulatory subunit. If structural studies require native protein ends, the GST tag can be removed.
4. GST-Tagged Protein Purification and Detection Assays
The N-terminal GST tag helps develop GST-based detection and purification methods for studying PSMD11 in different experimental setups. Researchers can use glutathione-affinity chromatography to isolate PSMD11-containing complexes from cell extracts or in vitro reaction mixtures. The GST tag also allows direct detection in ELISA-based assays using anti-GST antibodies when quantitative measurements are needed.
