Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
40S ribosomal protein S18; HKE3; KE 3; Ke-3; Ke3; Rhabdomyosarcoma antigen MU-RMS-40.21; Ribosomal protein S18; rps18; RS18_HUMAN
Species
Homo sapiens (Human)
Expression Region
2-152aa
Target Protein Sequence
SLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Human RPS18 contains amino acids 2-152. This RPS18 protein is expected to have a theoretical molecular weight of 44.6 kDa. The RPS18 protein was expressed in e.coli. The N-terminal GST tag was fused into the coding gene segment of RPS18, making it easier to detect and purify the RPS18 recombinant protein in the later stages of expression and purification.
The human 40S ribosomal protein S18 (RPS18) is a constituent of the small subunit (40S) of the eukaryotic ribosome. RPS18 plays a fundamental role in protein synthesis. RPS18 contributes to the formation of the ribosomal subunit, aiding in the assembly and stabilization of the small ribosomal particle. The ribosome, composed of both small and large subunits, functions as a molecular machine for translating mRNA into proteins. Research on RPS18 delves into understanding its precise role in ribosomal function, protein synthesis, and its potential implications in cellular processes and diseases associated with aberrations in translation machinery.
