Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

RPS18

Uniprot No.

P62269

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

40S ribosomal protein S18; HKE3; KE 3; Ke-3; Ke3; Rhabdomyosarcoma antigen MU-RMS-40.21; Ribosomal protein S18; rps18; RS18_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-152aa

Target Protein Sequence

SLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

44.6kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human RPS18 contains amino acids 2-152. This RPS18 protein is expected to have a theoretical molecular weight of 44.6 kDa. The RPS18 protein was expressed in e.coli. The N-terminal GST tag was fused into the coding gene segment of RPS18, making it easier to detect and purify the RPS18 recombinant protein in the later stages of expression and purification.

The human 40S ribosomal protein S18 (RPS18) is a constituent of the small subunit (40S) of the eukaryotic ribosome. RPS18 plays a fundamental role in protein synthesis. RPS18 contributes to the formation of the ribosomal subunit, aiding in the assembly and stabilization of the small ribosomal particle. The ribosome, composed of both small and large subunits, functions as a molecular machine for translating mRNA into proteins. Research on RPS18 delves into understanding its precise role in ribosomal function, protein synthesis, and its potential implications in cellular processes and diseases associated with aberrations in translation machinery.

Target Background

Function

Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.

Gene References into Functions

Ribosomal protein S18e seems to act as a molecular staple fixing the 18S rRNA 3'-major domain core. PMID: 21256985
cofilin interacts with S18 protein at the actin-binding site PMID: 15532723

Subcellular Location

Cytoplasm.

Protein Families

Universal ribosomal protein uS13 family

Database Links

HGNC: 10401

OMIM: 180473

KEGG: hsa:6222

STRING: 9606.ENSP00000393241

UniGene: Hs.448854

Code	CSB-EP020385HU
Abbreviation	Recombinant Human RPS18 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human 40S ribosomal protein S18 (RPS18)

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