Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

RPSA

Uniprot No.

P08865

Research Area

Cardiovascular

Alternative Names

34/67 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin binding protein; Colon carcinoma laminin-binding protein; LAMBR; Laminin receptor 1; Laminin-binding protein precursor p40; LAMR 1; LamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; Multidrug resistance associated protein MGr1 Ag; Multidrug resistance associated protein MGr1Ag; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; Ribosomal Protein SA; rpsA; RSSA_HUMAN; SA

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-295aa

Target Protein Sequence

SGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.7 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer，50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human RPSA protein is encoded by a recombinant DNA that was cloned into the expression vector and then transformed into the E.coli that supports the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 10xHis tag and C-terminal Myc tag gene to the gene fragment coding for the 2-295aa of the human RPSA protein. After purification, the product is the recombinant human RPSA protein. This recombinant RPSA protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This RPSA protein ran to the molecular weight of about 35-40 kDa under SDS-PAGE condition. It may have applications in cardiovascular research.

RPSA protein is a ribosomal protein which encoded by RPSA gene in human. RPSA gene consists of seven exons, six of which correspond to the coding sequence. RPSA structure generally is divided in an N-domain (residues 1-209) and a C-domain (residues 210-295). The structure of the N-domain of RPSA is similar to those of prokaryotic RPS2. RPSA is originally discovered by methods of cellular biology. RPSA binds to proteins that are involved in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis.

Target Background

Function

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.; (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9.; (Microbial infection) Acts as a receptor for the Dengue virus.; (Microbial infection) Acts as a receptor for the Sindbis virus.; (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus.; (Microbial infection) Acts as a receptor for the pathogenic prion protein.; (Microbial infection) Acts as a receptor for bacteria.

Gene References into Functions

Mutations in RPSA exons can affect the translated or untranslated regions and can underlie Isolated congenital asplenia with complete or incomplete penetrance. PMID: 30072435
The binding of laminin-1 to 67LR causes initial signaling through PKA and Epac, which causes the internalization of 67LR, along with signaling enzymes, such as adenylyl cyclase, into early endosomes. This causes sustained signaling for protection against neuronal cell death induced by serum withdrawal. PMID: 29108990
We suggest that PrP(C) and its interactor, LR/37/67 kDa, could be potential therapeutic targets for schwannomas and other Merlin-deficient tumours. PMID: 28692055
Our results suggest that hypoxia-elicited c-Jun/activator protein 1 regulates 37-kDa laminin receptor precursor expression, which modulates migration and invasion of lung adenocarcinoma cells PMID: 28618937
Increased LRP levels correlate with the increased invasive and adhesive potential in early and late stage melanoma cells. PMID: 28118986
A polysaccharide from Pinellia ternata inhibits cell proliferation and metastasis in human cholangiocarcinoma cells by targeting of Cdc42 and 67kDa Laminin Receptor PMID: 27576948
67LR plays a considerable role in the development of colon cancer multidrug resistance PMID: 26293895
37LRP regulates the metastasis of glioma cells in vitro and tumor growth in vivo. PMID: 27748570
Higher molecular weight Rpsa requires sumoylation to form. PMID: 26146125
Knock-Down of the 37kDa/67kDa Laminin Receptor LRP/LR Impedes Telomerase Activity PMID: 26545108
High expression level of the laminin receptor is associated with Breast and Oesophageal Cancer. PMID: 26427016
Report discovery of new small molecules inhibiting 67 kDa laminin receptor interaction with laminin and cancer cell invasivness. PMID: 26062445
LR1 contributes to hypoxia-induced migration and invasion of trophoblast cells at least partly by mediating MMP-9 PMID: 25800042
Mutations in the gene RPSA, which encodes ribosomal protein SA, cause more than half of the cases of isolated congenital asplenia. These disease-causing mutations lead to haploinsufficiency of RPSA. Review. PMID: 25840456
analysis of the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis PMID: 25274119
Studies indicate that the expression of 37/67-kDa immature laminin receptor protein (iLRP)iLRP in the rodent and human fetus is phase specific PMID: 25082063
Suggest role for 67LR/mitogen activated protein kinase/DUSP pathway in influencing glioma cell proliferation. PMID: 25778325
a biological marker in distinguishing squamous cell carcinoma: from adenocarcinoma, neuroendocrine carcinomas, and malignant mesothelioma PMID: 24805133
LRP/LR is a receptor for amyloid-beta42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide. PMID: 24990253
Data suggest thet 67-kDa laminin receptor-dependent protein phosphatase 2A (67LR/PP2A) may be a promising therapeutic target for melanomas. PMID: 25294877
Results indicate that MGr1-Ag/37LRP contributes to laminin-mediated cell adhesion-mediated drug resistance in gastric cancer cells. PMID: 24840404
Mutants in the RPSA gene might be associated with genetic susceptibility to colorectal cancer. PMID: 24460263
LamR interaction with the yeast prion-forming protein, Sup35, was investigated. PMID: 24416454
as a receptor of ECM components, MGr1-Ag/37LRP may activate the downstream signal pathway PI3K/AKT and MAPK/ERK through interaction with phosphorylated FAK. PMID: 24703465
RNA interference-mediated silencing of laminin receptor 1 (LR1) suppresses migration and invasion and down-regulates matrix metalloproteinase (MMP)-2 and MMP-9 in trophoblast cells. PMID: 23729238
findings indicate that 37/67LR regulates proliferation and adhesion in normal intestinal epithelial cells independently of its known association with ribosomal function PMID: 23991217
PEDF causes anti-angiogenic, anti-inflammatory and anti-thrombogenic reactions in myeloma cells through the interaction with LR. PMID: 24342618
LRP/LR is involved in the maintenance of cellular viability in tumorigenic lung and cervix uteri cells through the blockage of apoptosis PMID: 23472084
MGr1-Ag promotes small-cell lung cancer cell invasion and bone metastasis in vitro and in vivo, and that this is partially mediated via the epithelial-mesenchymal transition pathway. PMID: 23588894
This discovery establishes an essential role for RPSA in human spleen development. PMID: 23579497
67LR functions as a cancer-specific death receptor. In this cell death receptor pathway, cGMP initiated cancer-specific cell death by activating the PKCdelta/acid sphingomyelinase (PKCdelta/ASM) pathway. PMID: 23348740
Identification of novel laminin receptor binding proteins from whole cell extracts. PMID: 22909348
The 67 kD laminin receptor is a novel PED/PEA-15 interacting protein. PED/PEA-15 overexpression increases 67LR-mediated cell adhesion and migration to laminin and extracellular matrix invasion. PMID: 21895963
results suggest that RNF8 and BRCA1 are anchored to the nucleolus through reversible interactions with RPSA PMID: 22814251
the nature of the EGCG-67LR interaction and novel structural insights into the understanding of 67LR-mediated functions of EGCG PMID: 22666419
a high plasticity of RPSA, which could be important for its multiple cellular localizations and functional interactions PMID: 22640394
key structural determinants of the interaction of LamR with laminin-1 PMID: 22290616
Data indicate that high iLR expression was strongly correlated with negativity for CD38 and ZAP-70 expression and mutated IGVH gene status. PMID: 21055809
Decreased LR1 expression in cytotrophoblasts and syncytiotrophoblasts of preeclamptic placentas, which may be independent of disease severity, might have a role in shallow trophoblastic invasion in preeclampsia. PMID: 21391874
The genotypes and allele frequencies of the RPSA polymorphisms showed no significant differences between the controls and sporadic CJD patients. PMID: 21838916
The ability of LAMR to regulate viability is associated with its C-terminal 75 residues. PMID: 21243100
the SA C-terminal domain in the spatial structure of the 40S subunit PMID: 21167900
67-kDa laminin receptor expression influenced the characteristics of leukemia cells toward an aggressive phenotype and increased the number of granulocyte-macrophage colony-stimulating factor receptors PMID: 21056082
Here, the authors identify a laminin binding site on LamR, comprising residues Phe32, Glu35, and Arg155, which are conserved among mammalian species. PMID: 21040730
67LR induces FasL expression and cytotoxicity against Fas-sensitive Jurkat T cells in human cholangiocarcinoma cells through the phosphorylation of c-Myc on Ser-62 and the subsequent activation of the FasL promoter through the ERK pathway PMID: 20101459
Studies indicate that the 37-kDa/67 kDa laminin receptor is a receptor for the cellular prion protein (PrPc). PMID: 20515747
67LR promotes the invasive and metastatic ability of the gastric cancer cells through increasing urokinase and MMP 9 expression. PMID: 20491781
TSAd associates with laminin binding protein and mediates T lymphocyte migration during T cell activation PMID: 19561400
Results suggested that the LBP receptor domain interacting with venezuelan equine encephalitis E2 and tick-borne encephalitis virus E viral proteins is located at the C-terminal fragment of the LBP molecule. PMID: 19961413
Tthese results show that 37LRP has some of the biological activities of 67LR, even prior to the conversion event. However, the conversion affects the sites of interaction with both laminin and heparan sulfate. PMID: 19691449

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Involvement in disease

Asplenia, isolated congenital (ICAS)

Subcellular Location

Cell membrane. Cytoplasm. Nucleus.

Protein Families

Universal ribosomal protein uS2 family

Database Links

HGNC: 6502

OMIM: 150370

KEGG: hsa:3921

STRING: 9606.ENSP00000346067

UniGene: Hs.449909

Code	CSB-EP020485HUb1
Abbreviation	Recombinant Human RPSA protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Small ribosomal subunit protein uS2 (RPSA)

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