Product Details

Uniprot No.

Target Names

RPSA

Alternative Names

34/67 kDa laminin receptor antibody; 37 kDa laminin receptor precursor antibody; 37/67 kDa laminin receptor antibody; 37LRP antibody; 40S ribosomal protein SA antibody; 67 kDa laminin receptor antibody; 67LR antibody; Colon carcinoma laminin binding protein antibody; Colon carcinoma laminin-binding protein antibody; LAMBR antibody; Laminin receptor 1 antibody; Laminin-binding protein precursor p40 antibody; LAMR 1 antibody; LamR antibody; LAMR1 antibody; LBP antibody; LBP/p40 antibody; LRP antibody; LRP/LR antibody; Multidrug resistance associated protein MGr1 Ag antibody; Multidrug resistance associated protein MGr1Ag antibody; Multidrug resistance-associated protein MGr1-Ag antibody; NEM/1CHD4 antibody; p40 antibody; Ribosomal Protein SA antibody; rpsA antibody; RSSA_HUMAN antibody; SA antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human RPSA

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8D9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RPSA, also known as the 37/67 kDa laminin receptor, serves a fascinating dual role in cellular biology. As a component of the 40S ribosomal subunit, it participates in protein synthesis, while its membrane-associated form functions as a receptor for laminin, mediating cell adhesion to the extracellular matrix. This multifunctional protein has garnered significant research attention due to its involvement in tumor cell invasion, metastasis, and its identification as a multidrug resistance-associated protein in cancer studies.

This recombinant monoclonal antibody, clone 8D9, offers the reproducibility and consistency that demanding research applications require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and ensures reliable performance across your experimental timeline.

Validation across multiple platforms demonstrates this antibody's versatility in your workflow. Western blot analysis confirms robust detection across an extensive panel of human cell lines including PC-3, U-251MG, K562, HeLa, A549, SW620, HepG2, and HEK293, as well as mouse NIH/3T3 cells, supporting the stated cross-species reactivity with human and mouse samples. The observed band at approximately 42 kDa, rather than the predicted 33 kDa molecular weight, reflects the well-documented post-translational modifications and dimerization behavior characteristic of this protein. Immunofluorescence staining in HepG2 cells reveals clear cytoplasmic and membrane localization patterns, while flow cytometry analysis in MCF-7 cells demonstrates distinct positive population shifts suitable for quantitative studies.

Whether investigating laminin-mediated cell adhesion, ribosomal function, or cancer biology, this antibody provides a reliable tool for advancing your research objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.; (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9.; (Microbial infection) Acts as a receptor for the Dengue virus.; (Microbial infection) Acts as a receptor for the Sindbis virus.; (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus.; (Microbial infection) Acts as a receptor for the pathogenic prion protein.; (Microbial infection) Acts as a receptor for bacteria.

Gene References into Functions

Mutations in RPSA exons can affect the translated or untranslated regions and can underlie Isolated congenital asplenia with complete or incomplete penetrance. PMID: 30072435
The binding of laminin-1 to 67LR causes initial signaling through PKA and Epac, which causes the internalization of 67LR, along with signaling enzymes, such as adenylyl cyclase, into early endosomes. This causes sustained signaling for protection against neuronal cell death induced by serum withdrawal. PMID: 29108990
We suggest that PrP(C) and its interactor, LR/37/67 kDa, could be potential therapeutic targets for schwannomas and other Merlin-deficient tumours. PMID: 28692055
Our results suggest that hypoxia-elicited c-Jun/activator protein 1 regulates 37-kDa laminin receptor precursor expression, which modulates migration and invasion of lung adenocarcinoma cells PMID: 28618937
Increased LRP levels correlate with the increased invasive and adhesive potential in early and late stage melanoma cells. PMID: 28118986
A polysaccharide from Pinellia ternata inhibits cell proliferation and metastasis in human cholangiocarcinoma cells by targeting of Cdc42 and 67kDa Laminin Receptor PMID: 27576948
67LR plays a considerable role in the development of colon cancer multidrug resistance PMID: 26293895
37LRP regulates the metastasis of glioma cells in vitro and tumor growth in vivo. PMID: 27748570
Higher molecular weight Rpsa requires sumoylation to form. PMID: 26146125
Knock-Down of the 37kDa/67kDa Laminin Receptor LRP/LR Impedes Telomerase Activity PMID: 26545108
High expression level of the laminin receptor is associated with Breast and Oesophageal Cancer. PMID: 26427016
Report discovery of new small molecules inhibiting 67 kDa laminin receptor interaction with laminin and cancer cell invasivness. PMID: 26062445
LR1 contributes to hypoxia-induced migration and invasion of trophoblast cells at least partly by mediating MMP-9 PMID: 25800042
Mutations in the gene RPSA, which encodes ribosomal protein SA, cause more than half of the cases of isolated congenital asplenia. These disease-causing mutations lead to haploinsufficiency of RPSA. Review. PMID: 25840456
analysis of the complex three-way interaction between the non-integrin laminin receptor, galectin-3 and Neisseria meningitidis PMID: 25274119
Studies indicate that the expression of 37/67-kDa immature laminin receptor protein (iLRP)iLRP in the rodent and human fetus is phase specific PMID: 25082063
Suggest role for 67LR/mitogen activated protein kinase/DUSP pathway in influencing glioma cell proliferation. PMID: 25778325
a biological marker in distinguishing squamous cell carcinoma: from adenocarcinoma, neuroendocrine carcinomas, and malignant mesothelioma PMID: 24805133
LRP/LR is a receptor for amyloid-beta42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide. PMID: 24990253
Data suggest thet 67-kDa laminin receptor-dependent protein phosphatase 2A (67LR/PP2A) may be a promising therapeutic target for melanomas. PMID: 25294877
Results indicate that MGr1-Ag/37LRP contributes to laminin-mediated cell adhesion-mediated drug resistance in gastric cancer cells. PMID: 24840404
Mutants in the RPSA gene might be associated with genetic susceptibility to colorectal cancer. PMID: 24460263
LamR interaction with the yeast prion-forming protein, Sup35, was investigated. PMID: 24416454
as a receptor of ECM components, MGr1-Ag/37LRP may activate the downstream signal pathway PI3K/AKT and MAPK/ERK through interaction with phosphorylated FAK. PMID: 24703465
RNA interference-mediated silencing of laminin receptor 1 (LR1) suppresses migration and invasion and down-regulates matrix metalloproteinase (MMP)-2 and MMP-9 in trophoblast cells. PMID: 23729238
findings indicate that 37/67LR regulates proliferation and adhesion in normal intestinal epithelial cells independently of its known association with ribosomal function PMID: 23991217
PEDF causes anti-angiogenic, anti-inflammatory and anti-thrombogenic reactions in myeloma cells through the interaction with LR. PMID: 24342618
LRP/LR is involved in the maintenance of cellular viability in tumorigenic lung and cervix uteri cells through the blockage of apoptosis PMID: 23472084
MGr1-Ag promotes small-cell lung cancer cell invasion and bone metastasis in vitro and in vivo, and that this is partially mediated via the epithelial-mesenchymal transition pathway. PMID: 23588894
This discovery establishes an essential role for RPSA in human spleen development. PMID: 23579497
67LR functions as a cancer-specific death receptor. In this cell death receptor pathway, cGMP initiated cancer-specific cell death by activating the PKCdelta/acid sphingomyelinase (PKCdelta/ASM) pathway. PMID: 23348740
Identification of novel laminin receptor binding proteins from whole cell extracts. PMID: 22909348
The 67 kD laminin receptor is a novel PED/PEA-15 interacting protein. PED/PEA-15 overexpression increases 67LR-mediated cell adhesion and migration to laminin and extracellular matrix invasion. PMID: 21895963
results suggest that RNF8 and BRCA1 are anchored to the nucleolus through reversible interactions with RPSA PMID: 22814251
the nature of the EGCG-67LR interaction and novel structural insights into the understanding of 67LR-mediated functions of EGCG PMID: 22666419
a high plasticity of RPSA, which could be important for its multiple cellular localizations and functional interactions PMID: 22640394
key structural determinants of the interaction of LamR with laminin-1 PMID: 22290616
Data indicate that high iLR expression was strongly correlated with negativity for CD38 and ZAP-70 expression and mutated IGVH gene status. PMID: 21055809
Decreased LR1 expression in cytotrophoblasts and syncytiotrophoblasts of preeclamptic placentas, which may be independent of disease severity, might have a role in shallow trophoblastic invasion in preeclampsia. PMID: 21391874
The genotypes and allele frequencies of the RPSA polymorphisms showed no significant differences between the controls and sporadic CJD patients. PMID: 21838916
The ability of LAMR to regulate viability is associated with its C-terminal 75 residues. PMID: 21243100
the SA C-terminal domain in the spatial structure of the 40S subunit PMID: 21167900
67-kDa laminin receptor expression influenced the characteristics of leukemia cells toward an aggressive phenotype and increased the number of granulocyte-macrophage colony-stimulating factor receptors PMID: 21056082
Here, the authors identify a laminin binding site on LamR, comprising residues Phe32, Glu35, and Arg155, which are conserved among mammalian species. PMID: 21040730
67LR induces FasL expression and cytotoxicity against Fas-sensitive Jurkat T cells in human cholangiocarcinoma cells through the phosphorylation of c-Myc on Ser-62 and the subsequent activation of the FasL promoter through the ERK pathway PMID: 20101459
Studies indicate that the 37-kDa/67 kDa laminin receptor is a receptor for the cellular prion protein (PrPc). PMID: 20515747
67LR promotes the invasive and metastatic ability of the gastric cancer cells through increasing urokinase and MMP 9 expression. PMID: 20491781
TSAd associates with laminin binding protein and mediates T lymphocyte migration during T cell activation PMID: 19561400
Results suggested that the LBP receptor domain interacting with venezuelan equine encephalitis E2 and tick-borne encephalitis virus E viral proteins is located at the C-terminal fragment of the LBP molecule. PMID: 19961413
Tthese results show that 37LRP has some of the biological activities of 67LR, even prior to the conversion event. However, the conversion affects the sites of interaction with both laminin and heparan sulfate. PMID: 19691449

Hide All

Involvement in disease

Asplenia, isolated congenital (ICAS)

Subcellular Location

Cell membrane. Cytoplasm. Nucleus.

Protein Families

Universal ribosomal protein uS2 family

Database Links

HGNC: 6502

OMIM: 150370

KEGG: hsa:3921

STRING: 9606.ENSP00000346067

UniGene: Hs.449909

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA164048A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: PC-3 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), K562 whole cell lysate(30µg), HeLa whole cell lysate(30µg), A549 whole cell lysate(30µg), SW620 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: RPSA antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 42 kDa Exposure time: 1min Immunofluorescence staining of HepG2 cell with CSB-RA164048A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA164048A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

RPSA Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background