Recombinant Human 45 kDa calcium-binding protein (SDF4)

Code CSB-YP861120HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP861120HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861120HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP861120HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SDF4
Uniprot No.
Alternative Names
45 kDa calcium-binding protein; Cab45; CAB45_HUMAN; Calcium binding protein; PSEC0034; SDF 4; SDF-4; SDF4; Stromal cell derived factor 4; Stromal cell-derived factor 4
Species
Homo sapiens (Human)
Expression Region
37-362
Target Protein Sequence
RPAN HSSTRERVAN REENEILPPD HLNGVKLEMD GHLNRGFHQE VFLGKDLGGF DEDAEPRRSR RKLMVIFSKV DVNTDRKISA KEMQRWIMEK TAEHFQEAME ESKTHFRAVD PDGDGHVSWD EYKVKFLASK GHSEKEVADA IRLNEELKVD EETQEVLENL KDRWYQADSP PADLLLTEEE FLSFLHPEHS RGMLRFMVKE IVRDLDQDGD KQLSVPEFIS LPVGTVENQQ GQDIDDNWVK DRKKEFEELI DSNHDGIVTA EELESYMDPM NEYNALNEAK QMIAVADENQ NHHLEPEEVL KYSEFFTGSK LVDYARSVHE EF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.; Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.
Gene References into Functions
  1. Ca(2+)-dependent changes in Cab45 mediate sorting of specific cargo molecules at the trans-Golgi network. PMID: 27138253
  2. Data reveal that the ability of Cab45S to inhibit SERCA2b activity is crucial for its role as a modulator of cell proliferation and tumor growth. PMID: 25772237
  3. The obtained results indicate that the changes in gene expression in bone marrow progenitor cells can be involved into space flight-induced osteopenia. PMID: 25509878
  4. Cab45S, a novel regulator of GRP78/BiP, suppresses endoplasmic reticulum stress-induced IRE1 activation and apoptosis by binding to and elevating GRP78/BiP, and has a role in the inhibition of ER stress-induced apoptosis. PMID: 24810055
  5. Calcium is required for the retention of Cab45 in the Golgi membranes, where Cab45 binds secretory cargo in a calcium-dependent reaction. PMID: 23266954
  6. In a yeast two-hybrid screen the EF-hand Ca(2+)-binding protein Cab45 was identified as an interaction partner of Munc18b. PMID: 17442889
  7. SDF-2, SDF-4 and SDF-5 are expressed in mammary tissues and cells and that a reduced level of SDF-2 and SDF-4 are associated with a poor clinical outcome. PMID: 19513569

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Subcellular Location
[Isoform 1]: Golgi apparatus lumen.; [Isoform 5]: Cytoplasm. Cell membrane. Cell projection, bleb.
Protein Families
CREC family
Tissue Specificity
Ubiquitous. Isoform 5 is expressed in pancreas.
Database Links

HGNC: 24188

OMIM: 614282

KEGG: hsa:51150

STRING: 9606.ENSP00000353094

UniGene: Hs.42806

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