Recombinant Human 60S ribosomal protein L15 (RPL15)

Code CSB-YP020144HU
MSDS
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Source Yeast
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Code CSB-EP020144HU
MSDS
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Source E.coli
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Code CSB-EP020144HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020144HU
MSDS
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Source Baculovirus
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Code CSB-MP020144HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RPL15
Uniprot No.
Alternative Names
60S ribosomal protein L15; EC45; FLJ26304; ribosomal protein L15 ; RL15_HUMAN; RPL10; RPL15; RPLY10 ; RPYL10
Species
Homo sapiens (Human)
Expression Region
2-204aa
Target Protein Sequence
GAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. Aurora kinase inhibitor danusertib negatively regulated AURKB/p70S6K/RPL15 axis with the involvement of PI3K/Akt/mTOR, AMPK, and p38 MAPK signaling pathways, leading to the induction of apoptosis and autophagy in human leukemia cells. PMID: 27612557
  2. Downregulation of RPL15 is associated with tumor progression in pancreatic ductal adenocarcinoma. PMID: 26498693
  3. These data identify RPL15 as a new gene involved in Diamond-Blackfan anemia. PMID: 23812780
  4. ISG56 interacts with ribosomal protein L15 in gastric cancer cells. PMID: 21612406
  5. RPL15 gene expression is decreased in both classic and follicular variants of papillary thyroid carcinoma. PMID: 21509594
  6. RPL15 promotes cell proliferation in gastric neoplasms PMID: 16608517

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Involvement in disease
Diamond-Blackfan anemia 12 (DBA12)
Subcellular Location
Membrane; Lipid-anchor.
Protein Families
Eukaryotic ribosomal protein eL15 family
Database Links

HGNC: 10306

OMIM: 604174

KEGG: hsa:6138

STRING: 9606.ENSP00000309334

UniGene: Hs.381219

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