Recombinant Human 60S ribosomal protein L5 (RPL5)

Code CSB-RP024954h
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
RPL5
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
60S ribosomal protein L5; DBA6; L5; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; Ribosomal protein L5; RL5; RL5_HUMAN; RP L5; RPL 5; RPL5; U21 RNA; U21RNA
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
2-297aa
Target Protein Sequence
GFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
61.2kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.
Gene References into Functions
  1. RPL5 remains an interesting candidate in multiple myelom(MM )because it is deleted in 20-40% of MM cases PMID: 28428269
  2. Whole exome sequencing in the differential diagnosis of Diamond-Blackfan anemia: Clinical and molecular study of three patients with novel RPL5 and mosaic RPS19 mutations PMID: 28376382
  3. Low RPL5 expression is associated with cancer. PMID: 28147343
  4. Ribosomal proteins L11 and L5 activate TAp73 by overcoming MDM2 inhibition. PMID: 25301064
  5. RPL5 mutation is associated with Diamond Blackfan Anemia. PMID: 25132370
  6. Findings uncover a mechanism by which RPL5 and RPL11 can co-operatively suppress c-Myc expression, allowing a tightly controlled ribosome biogenesis in cells. PMID: 24141778
  7. Unlike other tumor suppressors, RPL5 and RPL11 play essential roles in normal cell proliferation. PMID: 24061479
  8. High frequency of RPL5 gene deletion is associated with Italian Diamond-Blackfan anemia. PMID: 22689679
  9. Oncogenic splicing factor SRSF1 stabilizes the tumor suppressor protein p53 via RPL5, inducing cell senescence. PMID: 23478443
  10. Mutations affect the ribosomal proteins RPL5 and RPL10 in 12 of 122 (9.8%) pediatric T-cell acute lymphoblastic leukemias. PMID: 23263491
  11. disrupted nucleoli may provide a platform for L5- and L11-dependent p53 activation, implying a role for the nucleolus in p53 activation by ribosomal biogenesis stress PMID: 23169665
  12. Data show 1 proband with an RPL5 deletion, 1 patient with an RPL35A deletion, 3 with RPS17 deletions, and 1 with an RPS19 deletion. PMID: 22262766
  13. Data show that all patients with RPS19 and RPL5 mutations had physical abnormalities. PMID: 20378560
  14. An analysis and fine mapping of GFI-EVI5-RPL5-FAM69A locus, genotyping eight Tag-single nucleotide polymorphisms in 732 multiple sclerosis patients and 974 controls from Spain, was performed. PMID: 20087403
  15. Knockdown of L29 or L30 enhanced the interaction of MDM2 with L11 and L5 and markedly inhibited MDM2-mediated p53 ubiquitination, suggesting that direct perturbation of 60 S ribosomal biogenesis activates p53 via L11- and L5-mediated MDM2 suppression. PMID: 20554519
  16. The study reports a high frequency of RPL5 (9.3%) and RPL11 (9.3%) mutations in a Diamond-Blackfan anemia cohort. PMID: 19773262
  17. the presence of multiple NLSs in ribosomal protein L5 appears to allow for efficient nuclear transport via utilisation of multiple, mechanistically different import pathways. PMID: 11824785
  18. the MDM2-L5-L11-L23 complex functions to inhibit MDM2-mediated p53 ubiquitination and thus activates p53 PMID: 15308643
  19. interaction of NVL2 with L5 is ATP-dependent and likely contributes to the nucleolar translocation of NVL2 PMID: 15469983
  20. Cancer-associated missense mutations targeting MDM2's central zinc finger disrupt the interaction of MDM2 with L5 and L11. PMID: 17116689
  21. 5-FU treatment triggers a ribosomal stress response so that ribosomal proteins L5, L11, and L23 are released from ribosome to activate p53 by ablating the MDM2-p53 feedback circuit PMID: 17242401
  22. L11 cooperates with L5, resulting in a robust inhibition of the E3 activity of MDM2, and a stabilization and activation of p53 approaching that achieved by p14(ARF). PMID: 18560357
  23. Susceptibility gene for multiple sclerosis in Australians. PMID: 18650830
  24. RPL5 mutations are associated with cleft palate and abnormal thumbs in Diamond-Blackfan anemia patients. PMID: 19061985
  25. Mutations in RPL5 were identified in eight patients from 6 out of 28 families (21.4%). PMID: 19191325

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Involvement in disease
Diamond-Blackfan anemia 6 (DBA6)
Subcellular Location
Cytoplasm. Nucleus, nucleolus.
Protein Families
Universal ribosomal protein uL18 family
Database Links

HGNC: 10360

OMIM: 603634

KEGG: hsa:6125

STRING: 9606.ENSP00000359345

UniGene: Hs.532359

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