Recombinant Human 60S ribosome subunit biogenesis protein NIP7 homolog (NIP7)

Code CSB-EP897077HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
NIP7
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
60S ribosome subunit biogenesis protein NIP7 homolog; CGI 37; CGI37; FLJ10296; HSPC031; HSPC180; KD93; NIP 7; NIP7; NIP7 nucleolar pre rRNA processing protein; NIP7_HUMAN; Nuclear import 7; Nuclear import 7 homolog (S. cerevisiae); Nuclear import 7 homolog; OK/SW cl.76; OK/SW cl.78; OTTHUMP00000174889
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-180aa
Target Protein Sequence
MRPLTEEETRVMFEKIAKYIGENLQLLVDRPDGTYCFRLHNDRVYYVSEKIMKLAANISGDKLVSLGTCFGKFTKTHKFRLHVTALDYLAPYAKYKVWIKPGAEQSFLYGNHVLKSGLGRITENTSQYQGVVVYSMADIPLGFGVAAKSTQDCRKVDPMAIVVFHQADIGEYVRHEETLT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
36.5kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.
Gene References into Functions
  1. The results presented in this work indicate a close functional interaction between NIP7 and FTSJ3 during pre-rRNA processing and show that FTSJ3 participates in ribosome synthesis in human cells. PMID: 22195017
  2. Downregulation of NIP7 affects pre-rRNA processing, causing an imbalance of the 40S/60S subunit ratio. PMID: 20798176
  3. KD93 is a novel protein expressed in human hematopoietic stem/progenitor cells PMID: 15522784
Subcellular Location
Nucleus, nucleolus.
Protein Families
NIP7 family
Tissue Specificity
Expressed in hematopoietic stem/progenitor cells.
Database Links

HGNC: 24328

KEGG: hsa:51388

STRING: 9606.ENSP00000254940

UniGene: Hs.585728

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