Recombinant Human 80 kDa MCM3-associated protein (MCM3AP), partial

Code CSB-YP013592HU
MSDS
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Source Yeast
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Code CSB-EP013592HU
MSDS
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Source E.coli
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Code CSB-EP013592HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013592HU
MSDS
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Source Baculovirus
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Code CSB-MP013592HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MCM3AP
Uniprot No.
Alternative Names
80 kDa MCM3 associated protein; 80 kDa MCM3-associated protein; FLJ44336; FLJ45306; Germinal center associated nuclear protein; KIAA0572; MAP80; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; MCM3A; MCM3A_HUMAN; Mcm3ap; Minichromosome maintenance complex component 3 associated protein; Minichromosome maintenance deficient 3 associated protein; Minichromosome maintenance protein 3 associated protein ; Protein GANP; SAC3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.; Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.
Gene References into Functions
  1. The identification of MCM3AP variants in affected individuals from multiple centres establishes it as a disease gene for childhood-onset recessively inherited Charcot-Marie-Tooth neuropathy with intellectual disability. PMID: 28633435
  2. MCM3AP and POMP Mutations Cause a DNA-Repair and DNA-Damage-Signaling Defect in an Immunodeficient Child PMID: 26615982
  3. These results indicated that the GANP protein is associated with breast cancer resistance. PMID: 26749495
  4. GANP is induced in activated T4 cells & physically interacts with A3G. GANP is encapsidated in HIV-1 virions & modulates A3G packaging into the cores. Upregulation increases A3G-catalyzed viral G-->A hypermutation, suppressing infectivity. PMID: 24198285
  5. A homozygous potentially pathogenic variant (c.2743G>A) was identified, which encodes amino acid Lys, instead of conserved Glu, at the position 915, in patients with mild intellectual disability. PMID: 24123876
  6. GANP-mediated chromatin modification promotes transcription complex recruitment and positioning at immunoglobulin variable loci to favour AID targeting. PMID: 23652018
  7. The cellular protein MCM3AP is required for inhibition of cellular DNA synthesis by the IE86 protein of human cytomegalovirus. PMID: 23094019
  8. HBV DNA integration sites into human genome were random, and MCM3AP was a new site. PMID: 20714864
  9. GANP, a homologue of yeast Sac3 that is involved in mRNA export, is indispensable for ensuring the stability of human genomic DNA and GANP knockdown causes apoptosis and necrosis of p53-insufficient cancer cells. PMID: 22395445
  10. GANP transgene may play a critical role in Lyn tyrosine-protein kinase-mediated signaling during selection of high-affinity B cells in peripheral lymphoid organs. PMID: 22942428
  11. MCM3AP and GANP are different proteins, occupying different locations in the cell and transcribed from different promoters PMID: 21195085
  12. Data show that human germinal center-associated nuclear protein (GANP) is critically involved in cell proliferation at the mitotic phase through its selective support of shugoshin-1 mRNA export. PMID: 20384790
  13. GANP may serve as an essential link required to transport AID to B-cell nuclei and to target AID to actively transcribed IgV regions PMID: 20507984
  14. GANP depletion inhibits mRNA export, with retention of mRNPs and NXF1 in punctate foci within the nucleus. PMID: 20005110
  15. inhibits initiation of DNA replication via binding to MCM3 PMID: 12226073
  16. plays a certain important role in the maturation of immunoglobulin or selection of B cells in germinal centers during the immune response to TD-Ag. A selective function of GANP molecule on B cell proliferation and differentiation might exist. PMID: 12885157
  17. abnormal over-expression of GANP together with AID might be associated with rigorous DNA damage, potentially causing the malignant development of Cholangiocarcinoma (CCAs) during long-term inflammation. PMID: 19578742
  18. GANP protects cells from cellular senescence caused by DNA damage and that a significant decrease in GANP expression leads to malignancy by generating hyperploidy and chromosomal instability (CIN). PMID: 19686285

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Subcellular Location
[Isoform GANP]: Nucleus envelope. Nucleus, nuclear pore complex. Nucleus, nucleoplasm. Chromosome.; [Isoform MCM3AP]: Cytoplasm. Nucleus.
Protein Families
SAC3 family
Tissue Specificity
Widely expressed. Up-regulated in germinal center B-cells in tonsils (at protein level).
Database Links

HGNC: 6946

OMIM: 603294

KEGG: hsa:8888

STRING: 9606.ENSP00000291688

UniGene: Hs.389037

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