Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ADAMTS7

Uniprot No.

Q9UKP4

Research Area

Cell Biology

Alternative Names

A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7; A disintegrin like and metalloprotease with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein; ADAM TS 7; ADAM TS7; ADAM-TS 7; ADAM-TS7; ADAMTS 7; ADAMTS-7; Adamts7; ATS7_HUMAN; COMPase; DKFZp434H204

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

242-593aa

Target Protein Sequence

KWVETLVVADAKMVEYHGQPQVESYVLTIMNMVAGLFHDPSIGNPIHITIVRLVLLEDEEEDLKITHHADNTLKSFCKWQKSINMKGDAHPLHHDTAILLTRKDLCAAMNRPCETLGLSHVAGMCQPHRSCSINEDTGLPLAFTVAHELGHSFGIQHDGSGNDCEPVGKRPFIMSPQLLYDAAPLTWSRCSRQYITRFLDRGWGLCLDDPPAKDIIDFPSVPPGVLYDVSHQCRLQYGAYSAFCEDMDNVCHTLWCSVGTTCHSKLDAAVDGTRCGENKWCLSGECVPVGFRPEAVDGGWSGWSAWSICSRSCGMGVQSAERQCTQPTPKYKGRYCVGERKRFRLCNLQACP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

52.1 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Expressing the recombinant Human ADAMTS7 protein in e.coli cells involves inserting a DNA fragment encoding the Human ADAMTS7 protein (242-593aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the ADAMTS7 protein. The protein carries a N-terminal 6xHis-SUMO tag. The cells are lysed to collect the recombinant Human ADAMTS7 protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human ADAMTS7 protein exceeds 90%.

Target Background

Function

Metalloprotease that may play a role in the degradation of COMP.

Gene References into Functions

data show that ADAMTS-7 is associated with a vulnerable plaque phenotype in human carotid lesions. These data support previous observations of a potential proatherogenic role of ADAMTS-7. PMID: 28623250
Multivariate analysis showed that DeltaADAMTS-7(day 7 minus day 1) was independently associated with left ventricular reverse remodeling PMID: 29523183
Genetic variation at the ADAMTS7 locus is associated with reduced severity of coronary artery disease. PMID: 29089340
Studied gene expression of genetic variants of ADAMTS7 in atherosclerotic occlusive peripheral arterial disease (PAD). Found mRNA levels of ADAMTS7 to be significantly higher in PAD patients than controls, and that the rs1994016 CC and rs3825807 TT genotypes may upregulate ADAMTS7 mRNA levels and may influence PAD development. PMID: 28205274
The findings suggest that upregulation of ADAMTS-7 and down regulation of COMP are associated with human AA. PMID: 28849199
The native overfunctional ADAMTS7 allele (A) may accelerate VSMC migration and lead to neointimal thickening, atherosclerosis progression and acute plaque events. PMID: 27614204
miR-105/Runx2 axis mediates FGF2-induced ADAMTS expression in osteoarthritis cartilage. PMID: 26816250
Allelic variation that associates with reduced ADAMTS7 expression confers stronger coronary heart disease protection in never-smokers than in ever-smokers. PMID: 28461624
During inflammatory conditions, AP-1 and Sp1 sustained the expression of ADAMTS7, and ADAMTS7 sustained the expression of catabolic genes in nucleus pulposus cells PMID: 27516213
ADAMTS7 and LPA single nucleotide polymorphisms are related to a 24-h ambulatory systolic-diastolic pressure regression index. PMID: 28092973
Expression of miR-26a and miR-29a was significantly down regulated in leukoplakia and cancer tissues but up regulated in lichen planus tissues. Expression of target genes such as, ADAMTS7, ATP1B1, COL4A2, CPEB3, CDK6, DNMT3a and PI3KR1 was significantly down regulated in at least two of three disease types with respect to normal tissues. PMID: 27515006
Our results indicate the presence of ADAMTS-7 in human NP cells and imply its potential role in disc degeneration. PMID: 26446668
The main contribution of this study is the proposal of a pharmacophore for ADAMTS7. PMID: 26872430
The significant associations observed between this coding variant in ADAMTS7 and the risk of CAD development. PMID: 26189211
Logistic regression analysis indicated that the association between ADAMTS-7 and heart failure after AMI was independent from traditional cardiovascular risk factors and other biomarkers PMID: 25885961
Data conclude that ADAMTS-7 level appears to be positively associated with expression of TNF-alpha and Phospho-NF-kappaB P65 in cartilage, which may imply its association with cartilage destruction of ONFH. PMID: 25653475
ADAMTS7 localized to cells having smooth muscle cell markers in human coronary artery disease lesions. Cultured vascular smooth muscle cells had ADAMTS7 at the cytoplasm and cell membrane, where it colocalized with markers of podosomes. PMID: 25712206
There was a reduction in the amount of cleaved ADAMTS7 prodomain in media conditioned by VSMCs of the G/G genotype. PMID: 23415669
statistically significant increase in mRNA expression of ADAMTS-7 and ADAMTS-12 was observed in the endplate cells in degenerative discs compared with nondegenerative discs PMID: 22247065
identified ADAMTS7 as novel locus for CAD and association of ABO with MI in the presence of CAD PMID: 21239051
ADAMTS-7 is the first metalloproteinase found to bind directly to and degrade COMP PMID: 16585064
ADAMTS-7 and ADAMTS-12 are newly identified enzymes responsible for cartilage oligomeric matrix protein degradation in arthritis. PMID: 19098927
Findings demonstrate that ADAMTS-7, a direct target of PTHrP signaling, negatively regulates endochondral bone formation by associating with and inactivating GEP chondrogenic growth factor. PMID: 19487464

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.

Database Links

HGNC: 223

OMIM: 605009

KEGG: hsa:11173

STRING: 9606.ENSP00000373472

UniGene: Hs.16441

Code	CSB-EP891951HU
Abbreviation	Recombinant Human ADAMTS7 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS7), partial

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