Recombinant Human AP-3 complex subunit delta-1 (AP3D1), partial

Code CSB-YP001876HU
MSDS
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Source Yeast
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Code CSB-EP001876HU
MSDS
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Source E.coli
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Code CSB-EP001876HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001876HU
MSDS
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Source Baculovirus
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Code CSB-MP001876HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AP3D1
Uniprot No.
Alternative Names
Adapter-related protein complex 3 subunit delta-1; adaptor-related protein complex 3, delta 1 subunit; ADTD; AP 3 complex delta subunit; AP-3 complex subunit delta; AP-3 complex subunit delta-1; ap3d1; AP3D1_HUMAN; Delta 1 subunit of adaptor-related protein complex 3; Delta Adaptin; Delta-adaptin; hBLVR; Subunit of putative vesicle coat adaptor complex AP 3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Involved in process of CD8+ T-cell and NK cell degranulation. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Gene References into Functions
  1. Unexpectedly, no evidence of binding between the N-terminal matrix (MA) domain of HIV-1 Gag and the cellular Adaptor Protein AP-3 (AP-3delta) was observed in these in vitro experiments. PMID: 22705971
  2. The matrix region of HIV-1 Gag interacts directly with the delta subunit of the AP-3 complex, and that this interaction plays an important functional role in particle assembly PMID: 15766529
  3. AP-3 and AP-1 function in partially redundant pathways to transfer tyrosinase from distinct endosomal subdomains to melanosomes and that the AP-3 pathway ensures that tyrosinase averts entrapment on internal membranes of forming multivesicular bodies PMID: 16162817
  4. The studies demonstrate a role for AP-3 in HIV replication in a tetraspanin-rich compartment in dendritic cells (DC) and contribute to the elucidation of the trafficking pathways required for DC-T cell transfer of HIV-1 infection. PMID: 18034776
Involvement in disease
Hermansky-Pudlak syndrome 10 (HPS10)
Subcellular Location
Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Adaptor complexes large subunit family
Tissue Specificity
Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis.
Database Links

HGNC: 568

OMIM: 607246

KEGG: hsa:8943

STRING: 9606.ENSP00000344055

UniGene: Hs.512815

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